344146
FPTase, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli
FPTase, GST-Fusion, His•Tag, Rat, Recombinant, E. coli, is composed of an74 kDa α- and 47 kDa β-subunit. The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag sequence.
別名:
FTase, Farnesyl Proteintransferase
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この商品について
NACRES:
NA.54
UNSPSC Code:
12352202
Assay:
≥90% (SDS-PAGE)
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
Quality Segment
assay
≥90% (SDS-PAGE)
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
General description
Recombinant, rat FPTase composed of an α- and a β-subunit (74 and 46 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag
Biochem/physiol Actions
1 pmol FTase will transfer ≥1 pmol farnesyl to H-Ras in 10 min at 37°C, pH 7.2.
Physical form
In 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Zimmerman, K.K., et al. 1998. Protein Expr. Purif.14, 395.
Reiss, Y., et al. 1990. Cell62, 81.
Reiss, Y., et al. 1990. Cell62, 81.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
保管分類
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable