362185
PNGase F Glycosidase
Removes all N-linked glycans, suitable for mass spectrometry, from Elizabethkingia meningosepticum
別名:
N-Glycosidase F, Elizabethkingia meningosepticum, Glycopeptidase F, PNGase F
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この商品について
CAS番号:
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
Specific activity:
≥20,000 units/mg protein, ≥4500 units/mL
Biological source:
bacterial (Elizabethkingia meningosepticum)
製品名
N-Glycosidase F, Elizabethkingia meningosepticum,
biological source
bacterial (Elizabethkingia meningosepticum)
Quality Segment
conjugate
(N-linked)
form
liquid
specific activity
≥20,000 units/mg protein, ≥4500 units/mL
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
foreign activity
Proteases, none detected
shipped in
wet ice
storage temp.
2-8°C
General description
Native N-Glycosidase F from Elizabethkingia meningosepticum. Catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins. This enzyme will not cleave oligosaccharides containing α1,3-linked core fucose commonly found on plant glycoproteins. N-Glycosidase F will also fail to cleave if the asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue at both the amino and carboxy termini.
Physical form
In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.
Other Notes
Means, R.E. and Desrosiers, R.C. 2000. J. Virol.74, 11181.
Fan, J.Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.
Fan, J.Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.
One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 nmol denatured ribonuclease B per min at 37°C, pH 7.5. Cleavage is monitored by SDS-PAGE. One unit of N-Glycosidase F is equal to 1 IUB milliunit.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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保管分類
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable