374805
Hemocyanin, Keyhole Limpet, Megathura crenulata
Hemocyanin, Keyhole Limpet, Megathura crenulata, CAS 9013-72-3, is a large, multi-subunit, oxygen-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.
別名:
Hemocyanin, Keyhole Limpet, Megathura crenulata, KLH
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この商品について
Quality Segment
description
Merck USA index - 14, 4647
assay
≥90% (size exclusion chromatography)
form
lyophilized solid
does not contain
preservative
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
blue to blue-gray
shipped in
ambient
storage temp.
2-8°C
General description
Hemocyanin purified from Keyhole Limpet (Mega thura crenulata). Protein:≥60%; copper to protein ratio: ≥1.5 x 10-3
Protein:≥60%; copper to protein ratio: ≥1.5 x 10-3
Stable solid containing more than 60% protein. Protein is 90% undissociated hemocyanin.
Physical form
Lyophilized from BES buffer, MgSO₄.
Preparation Note
Dissolve at 5-10 mg/ml (protein) in PBS or other physiological buffer by GENTLE addition of the buffer (e.g. slowly added down the side of the tube). DO NOT vortex or agitate vigorously to dissolve product. (KLH is an extremely large protein complex and has a tendency to aggregate and precipitate from solutions.) For further dilutions, use an appropriate buffer and maintain the magnesium concentration at ≥10 mM. Solutions should be stored in the refrigerator (4°C).
Other Notes
Hayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol. 63, 37.
Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137.
Erlanger, B.F. 1980. Methods Enzymol.70, 85.
Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137.
Erlanger, B.F. 1980. Methods Enzymol.70, 85.
Protein concentration: ≥60%
Copper: ≥0.09%
Copper to Protein ratio: ≥1.5 x 10-3
Copper: ≥0.09%
Copper to Protein ratio: ≥1.5 x 10-3
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
保管分類
11 - Combustible Solids
wgk
WGK 3
試験成績書（COA）
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