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Merck

374817

Hemocyanin, Keyhole Limpet, Megathura crenulata, Glycerol

Hemocyanin, Keyhole Limpet, Megathura crenulata, Glycerol, is a large, multi-subunit, oxygen-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.

別名:

Hemocyanin, Keyhole Limpet, Megathura crenulata, Glycerol, KLH

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この商品について

CAS番号:
UNSPSC Code:
12352202
NACRES:
NA.25
MDL number:
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Quality Segment

description

Merck USA index - 14, 4647

assay

>90% (size exclusion chromatography)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

clear blue-gray

shipped in

ambient

storage temp.

2-8°C

General description

Carrier protein that has many available primary amines to facilitate protein conjugation. Used to immunize animals to elicit antibodies to small molecules (haptens) by covalent conjugation. Protein: ≥10 mg/ml. Sold on the basis of protein content.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 10 mM MgSO₄, 5 mM BES, 50% glycerol, 0.9% sodium chloride, pH 6.5.

Preparation Note

Further dilute with PBS or physiological saline. Material can be dialyzed to remove the glycerol or used as is for conjugation with glutaraldehyde, carbodiimide, or mixed anhydrides.KLH is an extremely large protein complex that has a tendency to aggregate and precipitate from solutions. Use a buffer when diluting KLH and maintain the magnesium concentration at ≥10 mM. Store KLH solutions at 4°C. DO NOT freeze, vortex or agitate to dissolve KLH. Freezing or agitating KLH will cause protein aggregation and precipitation.

Other Notes

Hayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol.63, 37.
Shuler, K.R., et al. 1992. J. Immunol. Methods156, 137.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)


保管分類

10 - Combustible liquids

wgk

WGK 2



試験成績書(COA)

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