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382065

Bisbenzimide H 33342 Fluorochrome, Trihydrochloride

Name: Bisbenzimide H 33342 Fluorochrome, Trihydrochloride
Brand: MM

Cell-permeable, adenine-thymine-specific fluorescent stain.

別名:

Bisbenzimide H 33342 Fluorochrome, Trihydrochloride, 2ʹ-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5ʹ-bi-1H-benzimidazole, 3HCl, HOE 33342

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この商品について

実験式（ヒル表記法）:

C₂₇H₂₈N₆O · 3HCl

CAS番号:

875756-97-1

分子量:

561.93

MDL number:

MFCD00166996

UNSPSC Code:

12352111

NACRES:

NA.21

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特性

Quality Segment

100

description

RTECS - DT4200000

assay

≥95% (TLC)

form

solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, protect from light

color

yellow

solubility

water: 50 mg/mL

fluorescence

λ_ex 346 nm, λ_em 460 nm

shipped in

ambient

storage temp.

2-8°C

SMILES string

N1(CCN(CC1)c2cc3nc([nH]c3cc2)c4cc5nc([nH]c5cc4)c6ccc(cc6)OCC)C

InChI

1S/C27H28N6O/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31)

InChI key

PRDFBSVERLRRMY-UHFFFAOYSA-N

詳細

General description

Cell-permeable adenine-thymidine specific fluorescent stain. Useful for staining DNA, chromosomes or nuclei for fluorescence microscopy and flow cytometry applications. DNA-bound dye has an excitation maximum of 358 nm and an emission maximum of 460 nm. Useful for the simultaneous study of the cell cycle and apoptosis in unfixed cells. Induces cell differentiation. H33342 and the related compound H33258 (Cat. No. 382061) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs. Exhibits a 10 fold greater cell-permeability than H 33258.

Cell-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei for fluorescence microscopy and flow cytometry applications. Useful for the simultaneous study of the cell cycle and apoptosis in unfixed cells. Induces cell differentiation. H 33342 and the related compound H 33258 (Cat. No. 382061) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs. Exhibits a 10-fold greater cell-permeability than H 33258.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Adenine-thymine-specific fluorescent stain

Product does not compete with ATP.

Reversible: no

Preparation Note

Following reconstitution, store in the refrigerator (4°C). Stock solutions (1 mg/ml) are stable for up to 2 weeks at 4°C. Precipitates in phosphate buffer solutions.

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Werstuck, G., and Green, M.R. 1998. Science 282, 296.
Paddenberg, R., et al. 1996. Eur. J. Cell Biol. 71, 105.
Filatov, M.V., et al. 1995. Mutat. Res. 327, 209.
Belloc, F., et al. 1994. Cytometry17, 59. Crissman, H.A. 1990. Methods Cell Biol. 33, 97.
Hinkley, R.E., et al. 1986. Dev. Biol.118, 148.
Sandhu, L.C., et al. 1985. Cytometry6, 191.
Frankfurt, O.S. 1983. Cytometry4, 216.
Lalande, M.E., et al. 1981. Proc. Natl. Acad. Sci. USA78, 363.
Visser, J.W.M. 1980. Flow CytometryIV, 86.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Toxic & Carcinogenic / Teratogenic (G)

保管分類

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c