386023
Anti-Hsp47 Mouse mAb (M16.10A1)
liquid, clone M16.10A1, Calbiochem®
別名:
Anti-Colligin
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この商品について
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
M16.10A1, monoclonal
Species reactivity:
mouse, porcine, chicken, canine, human, hamster, guinea pig, bovine, rat, monkey, sheep
Application:
—
Citations:
2
biological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
M16.10A1, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
mouse, porcine, chicken, canine, human, hamster, guinea pig, bovine, rat, monkey, sheep
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA
Enzyme Immunoassay
Immunoblotting (1 µg/mL, colorimetric)
Immunocytochemistry (1 µg/mL)
Immunofluorescence (5 µg/mL)
Immunoprecipitation (5 µg/mL)
Paraffin Sections )
isotype
IgG2b
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... SERPINH1(871)
General description
Anti-Hsp47, mouse monoclonal, clone M16.10A1, recognizes the ~47 kDa Hsp47 protein in wide variety of species. It is validated for use in ELISA, EIA, WB, ICC, IF, IP, and IHC (paraffin sections).
Protein G purified mouse monoclonal antibody. Recognizes the ~47 kDa Hsp47 protein.
Recognizes the ~47 kDa Hsp47 protein.
Immunogen
Hsp47 from L6 cells
Rat
Application
ELISA (see comments)
Enzyme Immunoassay (see comments)
Immunoblotting (1 µg/ml, colorimetric)
Immunocytochemistry (1 µg/ml)
Immunofluorescence (5 µg/ml)
Immunoprecipitation (5 µg/ml)
Paraffin Sections (see comments)
Enzyme Immunoassay (see comments)
Immunoblotting (1 µg/ml, colorimetric)
Immunocytochemistry (1 µg/ml)
Immunofluorescence (5 µg/ml)
Immunoprecipitation (5 µg/ml)
Paraffin Sections (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, 50% glycerol, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
3T3 cells, Human breast cancer tissue
3T3 cells, Human breast cancer tissue
Other Notes
Kuroda, K., et al. 1998. J. Invest. Dermatol.111, 1023.
Nandan, D., et al. 1998. Exp. Cell Res.179, 289.
Clark, E.P., et al. 1991. J. Biol. Chem.266, 17230.
Vaillancourt, J.P., and Cates, G.A. 1991. Biochem. J.274, 793.
Cates, G.A., et al. 1987. Biochem. Cell Biol. 65, 767.
Saga, S., et al. 1987. J. Cell Biol.105, 517.
Nagata, K., et al. 1986. J. Biol. Chem. 261, 7531.
Nandan, D., et al. 1998. Exp. Cell Res.179, 289.
Clark, E.P., et al. 1991. J. Biol. Chem.266, 17230.
Vaillancourt, J.P., and Cates, G.A. 1991. Biochem. J.274, 793.
Cates, G.A., et al. 1987. Biochem. Cell Biol. 65, 767.
Saga, S., et al. 1987. J. Cell Biol.105, 517.
Nagata, K., et al. 1986. J. Biol. Chem. 261, 7531.
This antibody has also been reported to work for ELISA and enzyme immunoassay. Variables associated with assay conditions will dictate the optimal dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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保管分類
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable