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386040

Anti-Hsp90 Mouse mAb (AC88)

liquid, clone AC88, Calbiochem®

Sinónimos:

Anti-Hsp82, Anti-Hsp85, Anti-Hsp89

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NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
AC88, monoclonal
Species reactivity:
-
Application:
—
Citations:
4
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biological source

mouse

Quality Segment

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

AC88, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity (predicted by homology)

all

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(Immunoblotting (1 µg/mL, colorimetric)
Immunoprecipitation
Immunocytochemistry )

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HSP90AA1(3320)

General description

Protein G purified mouse monoclonal antibody. Recognizes the ~90 kDa Hsp90 protein.
Recognizes the ~90 kDa Hsp90 protein.
This Anti-Hsp90 Mouse mAb (AC88) is validated for use in Immunoblotting, Immunoprecipitation, Immunocytochemistry for the detection of Hsp90.

Immunogen

Achyla ambisexualis
Hsp90 isolated from water mold, Achyla ambisexualis

Application

Immunoblotting (1 µg/ml, colorimetric)

Immunoprecipitation (see comments)

Immunocytochemistry (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 10 mM PBS, 50% glycerol, pH 7.2.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Heat shocked HeLa cells, Heat shocked 3T3 cells, Heat shocked PC-12 cells, Heat shocked CHO-K1, Heat shocked Rat-2 cells

Other Notes

Cross-reacts with glucocorticoid receptor in rodent. By immunoprecipitation, the antibody favors free Hsp90 over complexed Hsp90. The optimal dilution for immunoprecipitation is assay dependent and must be determined for each sample. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the optimal dilution.
Harry, J.L. et al. 1990. Development190, 305.
Minoo, P. et al. 1989 J. Cell. Biol.109, 1937.
Riehl, R.M., et al. 1985. Biochemistry 24, 6586.
Sanchez, E.R., et al. 1985. J. Biol. Chem. 260, 12398.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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