431525
L-Leucine Dehydrogenase, Bacillus cereus
別名:
L-Leucine Dehydrogenase, Bacillus cereus, L-LeuDH, L-Leucine:NAD +-oxidoreductase
で組織・契約価格をご覧ください。
サイズを選択してください
表示を変更する
この商品について
CAS番号:
UNSPSC Code:
12352202
NACRES:
NA.77
MDL number:
Specific activity:
≥10 units/mg protein, ≥50 units/mL
Biological source:
Bacillus sp. (Bacillus cereus)
biological source
Bacillus sp. (Bacillus cereus)
Quality Segment
form
liquid
specific activity
≥10 units/mg protein, ≥50 units/mL
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−20°C
General description
Native L-leucine dehydrogenase from Bacillus cereus. Purified enzyme that catalyzes the NAD-dependent conversion of L-leucine to α-ketoisocaproate. Suitable for the production of L-tertiary leucine amino acids. Has an optimal pH of 10.5-10.8 for L-leucine substrate and 9.4 for α-ketoisocaproate substrate. Has an optimal temperature of 65°C.
pH optimum 10.5-10.8 (L-leucine substrate) and 9.4 (α-ketoisocaproate substrate), temperature optimum 65°C.
Physical form
In 100 mM NaCl, 10 mM potassium phosphate buffer, 50% glycerol, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Kärst, U., et al. 1986. FEMS Microbiol. Lett. 37, 335.
Schütte, H., et al. 1985. Appl. Microbiol.Biotechnol.22, 306.
Schütte, H., et al. 1985. Appl. Microbiol.Biotechnol.22, 306.
One unit is defined as the amount of enzyme that will convert 1.0 µmol L-leucine to α-ketoisocaproate in the presence of NAD+ per min at 30°C, pH 10.7.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
保管分類
10 - Combustible liquids
wgk
WGK 2
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。