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Merck

444905

Anti-Mast Cell Tryptase Mouse mAb (AA1)

liquid, clone AA1, Calbiochem®

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この商品について

NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
AA1, monoclonal
Species reactivity:
monkey, canine, human, feline
Application:
Citations:
2
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biological source

mouse

Quality Segment

antibody form

purified antibody

antibody product type

primary antibodies

clone

AA1, monoclonal

form

liquid

does not contain

preservative

species reactivity

monkey, canine, human, feline

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(Frozen Sections (0.06-0.12 µg/mL)
Immunoblotting (1 µg/mL)
Paraffin Sections (0.06-0.12 µg/mL, heat pre-treatment required))

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TPSAB1(7177)

General description

Protein G purified mouse monoclonal antibody. Recognizes the ~32-38 kDa α- and β-isoforms of tryptase in mast cells.
Recognizes the 32-38 kDa α- and β-isoforms of tryptase in mast cells.
This Anti-Mast Cell Tryptase Mouse mAb (AA1) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of Mast Cell Tryptase.

Immunogen

Human
native, human mast cell, tryptase

Application

Frozen Sections (0.06-0.12 µg/ml)

Immunoblotting (1 µg/ml)

Paraffin Sections (0.06-0.12 µg/ml, heat pre-treatment required, see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 10 mM PBS, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Cat, dog, human, or monkey mast cells

Other Notes

Sommerhoff, C.P., et al. 2000. Biochim. Biophys. Acta.1477, 75.
Little, S.S., and Johnson, D.A. 1995. Biochem. J.307, 341.
Morgan, S.J., et al. 1991. J. Allergy Clin. Immunol.87, 111.
Walls, A.F., et al. 1990. J. Pathol.162, 119.
Wall, A.F., et al. 1990. Clin. Exp. Allergy20, 581.
Staining of formalin-fixed paraffin sections requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min. Variables associated with assay conditions will dictate the optimal working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)


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保管分類

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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