Anti-Pan-NMDA NR1 Mouse mAb (R1JHL)

Mouse monoclonal antibody lyophilized from cell culture supernatant. Recognizes the ~115 kDa subunit of the NMDAR (N-methyl-D-aspartate receptor) protein.

Recognizes the ~115 kDa NMDA receptor NR1 subunit in rat cortex tissue extracts.

This Anti-Pan-NMDA NR1 Mouse mAb (R1JHL) is validated for use in Immunoblotting for the detection of Pan-NMDA NR1.

Rat

a recombinant fusion protein consisting of amino acids 1-564 of rat NMDA NR1

Immunoblotting (1:300, colorimetric)

Immunoprecipitation (3 µg/200 µg lysate)

Lyophilized from cell culture supernatant.

Reconstitute in 50 µl PBS (137 mM NaCl, 7.5 mM Na₂HPO₄, 2.7 mM KCl, 1.5 mM KH₂P0₄, pH 7.4)Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Positive Control
Rat cortex tissue extracts

Does not cross-react with other NMDAR subunits. For immunoblotting, dilute in TBST containing 5% nonfat milk and 0.05% sodium azide. Antibody may be used up to 5 times in immunoblotting experiments without significant loss in signal strength. Store diluted antibody at 4°C between uses. Variables associated with assay conditions will dictate the proper working dilution.

Laurie, D.J., and Seeburg, P.H. 1994. J. Neurosci.14, 3180
Ishii, T., et al. 1993. J. Biol. Chem.268, 2836.
Monyer, H., et al. 1992. Science256, 1217.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)