5.06028
Cell Proliferation Inducer, WS3
Synonym(s):
Cell Proliferation Inducer, WS3, N-(6-(4-(3-(4-((4-methylpiperazin-1-yl)methyl)-3-(trifluoromethyl)phenyl)ureido)phenoxy)pyrimidin-4-yl)cyclopropanecarboxamide Hydrochloride
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About This Item
Empirical Formula (Hill Notation):
C28H30F3N7O3 · xHCl
Molecular Weight:
569.58 (free base basis)
UNSPSC Code:
12352200
Assay:
≥98% (HPLC)
Form:
solid
Storage condition:
OK to freeze, protect from light
assay
≥98% (HPLC)
Quality Segment
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
color
tan
solubility
DMSO: 50 mg/mL, water: 50 mg/mL
storage temp.
2-8°C
General description
A cell-permeable, diarylurea analog that can reversibly induce cell proliferation of primary retinal pigment epithelial (RPE) cells from rodents and from fetal and adult human donors (~ 25 nM). Its action suggested to be mediated through Erb3 binding protein 1 (Ebp1). Non-proliferating RPE cells at passage 6 appear to respond well to WS3 treatment, whereas actively proliferating cells in passage 1 to 4 do not exhibit any increase in proliferation. These cells can differentiate into functional monolayer following WS3 withdrawal. However, WS3-expanded adult human RPE cells differentiate less effectively when compared to stem cell-derived RPE cells. The expanded RPE cells are shown to preserve vision when transplanted into rodent models of retinal degeneration. Also shown to induce proliferation of mouse R7T1 β cells (EC50 = 74 nM). Not reported to induce any genetic changes or tumorigenic events.
Biochem/physiol Actions
Reversible: yes
Packaging
Packaged under inert gas
Physical form
Supplied as a hydrochloride salt.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Swoboda, J.G., et al, 2013. ACS Chem. Biol.8, 1407.
Shen, W., et al, 2013. J. Am. Chem. Soc.135, 1669.
Shen, W., et al, 2013. J. Am. Chem. Soc.135, 1669.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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