PhosphoDetect Anti-p38 MAP Kinase (pThr¹⁸⁰, pTyr¹⁸²) Rabbit pAb

Note: 1 T = 1 test. Sufficient for 10 Western miniblots.

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~p38 MAPK phosphorylated at Thr¹⁸⁰ and Tyr¹⁸².

Recognizes p38α, β, and γ/ERK6 phosphorylated at Thr¹⁸⁰ and Tyr¹⁸² in uv-treated HEK293 cells and sorbitol-treated PC12 cells.

This PhosphoDetect Anti-p38 MAP Kinase Rabbit pAb is validated for use in WB, ICC for the detection of p38 MAP Kinase .

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸⁰ and Tyr¹⁸² phosphorylation sites of human p38 MAP Kinase

Immunoblotting (1:1000)

Immunocytochemistry (see comments)

In Dulbecco′s PBS (without Mg²⁺ and Ca²⁺), 1 mg/ml BSA, 50% glycerol, pH 7.3.

Following initial thaw, aliquot and freeze (-20°C).

Positive Control
UV irradiated HEK293 cells or sorbitol-treated PC12 cells

Does not appreciably recognize the corresponding phosphorylated forms of either p42/44 MAPK or SAPK/JNK MAPK. The immunogen sequence is 100% conserved in p38α, β, and γ. The immunogen sequence is also 100% conserved in mouse, moneky, and carp p38, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. When diluted 1:1000, there is sufficient antibody for 10 immunoblots at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.

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Toxicity: Standard Handling (A)