525295
PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20)
liquid, clone PY20, Calbiochem®
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この商品について
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
PY20, monoclonal
Species reactivity:
-
Application:
—
Citations:
5
biological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
PY20, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity (predicted by homology)
all
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA
Immunoblotting (1:500-1:1000,
Frozen Sections
Immunocytochemistry
Immunoprecipitation )
isotype
IgG2b
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pTyr)
Gene Information
human ... PID1(55022)
General description
Purified mouse monoclonal antibody. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states.
Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
This PhosphoDetect
Immunogen
KLH conjugated to phosphotyrosine, glycine, and alanine
Application
ELISA (see comments)
Immunoblotting (1:500-1:1000, see application references)
Frozen Sections (see application references)
Immunocytochemistry (see application references)
Immunoprecipitation (see comments)
Immunoblotting (1:500-1:1000, see application references)
Frozen Sections (see application references)
Immunocytochemistry (see application references)
Immunoprecipitation (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 10 mM sodium phosphate, pH 7.4.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Any cell lysate or tissue extract
Any cell lysate or tissue extract
Other Notes
Binding is inhibited by phosphotyrosine. Does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. This antibody has also been reported to work for ELISA and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Glenney, J.R., et al. 1988. J. Immunol. Methods109, 277.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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保管分類
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
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