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この商品について
NACRES:
NA.42
UNSPSC Code:
12352202
Specific activity:
≥25 units/mg protein
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
Quality Segment
form
liquid
specific activity
≥25 units/mg protein
manufacturer/tradename
Calbiochem®
shipped in
dry ice
storage temp.
−20°C
General description
Recombinant, human purine nucleotide phosphorylase expressed in E. coli without tags. Purine nucleoside phosphorylase catalyzes the phosphorlysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides in a reversible reaction. Human PNP is a target for therapeutic T-cell immune response intervention. Useful in the detection of inorganic phosphate from biochemical reactions.
Physical form
In 50 mM Tris-HCl, pH 7.6.
Analysis Note
Single band by SDS-PAGE
Other Notes
Filgueira de Azevedo, W.Jr., et al. 2003. Biochem. Biophys. Res. Commun.309, 917.
Silva, R.G., et al. 2003. Protein Expr. Purif.27, 158.
Silva, R.G., et al. 2003. Protein Expr. Purif.27, 158.
One unit is defined as the amount of enzyme that will convert 1 µmol of MESG and 1 µmol of Pi into 1 µmol of 2-amino-6-mercapto-7-methylpurine and 1 µmol of ribose phosphate in 1 min at 25°C, pH 7.6.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
保管分類
12 - Non Combustible Liquids
一体何が起きているの?
WGK 1
引火点 (F)
Not applicable
引火点(°C)
Not applicable