574777
Anti-Synapsin I Rabbit pAb
lyophilized, Calbiochem®
別名:
Rabbit Anti-Synapsin I, Synapsin I Detection Antibody
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この商品について
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
human, rat, mouse, bovine
Application:
—
Citations:
20
biological source
rabbit
Quality Segment
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
does not contain
preservative
species reactivity
human, rat, mouse, bovine
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(ELISA (1:2500-1:10,000)
Frozen Sections
Immunoblotting (1:200)
Immunocytochemistry (1:500-1:2500)
Immunoprecipitation (1 µg/200 µg lysate))
isotype
IgG
shipped in
ambient
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
mouse ... Syn1(20964)
General description
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the synapsin I protein.
Recognizes the synapsin I protein in rat brain.
This Anti-Synapsin I Rabbit pAb is validated for use in ELISA, Immunoblotting Immunocytochemistry Immunoprecipitation for the detection of Synapsin I.
Immunogen
Bovine
purified, brovine brain synapsin I
Application
ELISA (1:2500-1:10,000)
Frozen Sections (see application references)
Immunoblotting (1:200)
Immunocytochemistry (1:500-1:2500)
Immunoprecipitation (1 µg/200 µg lysate)
Frozen Sections (see application references)
Immunoblotting (1:200)
Immunocytochemistry (1:500-1:2500)
Immunoprecipitation (1 µg/200 µg lysate)
Physical form
Lyophilized from 5 mM ammonium bicarbonate.
Preparation Note
Reconstitute with 50 µl PBS. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Analysis Note
Positive Control
Rat brain
Rat brain
Other Notes
Stone, L.M., et al. 1994. J. Neurosci.14, 301.
Mandell, J.W., et al. 1992. J. Neurosci.12, 1736.
Fletcher, T.L., et al. 1991. J. Neurosci.11, 1617.
Moore, R.Y., and Berstein, M. 1989. J. Neurosci.9, 2151.
Mandell, J.W., et al. 1992. J. Neurosci.12, 1736.
Fletcher, T.L., et al. 1991. J. Neurosci.11, 1617.
Moore, R.Y., and Berstein, M. 1989. J. Neurosci.9, 2151.
This antibody is specific for synapsin I and can be used to estimate synaptic density and synaptogenesis. Strong and specific immunolabeling of the synapsin I doublet seen in immunoblotting at a 1:200 dilution. This immunolabeling can be blocked by preadsorption of antibody with synapsin I. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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