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676604

PhosphoDetect Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6)

Name: PhosphoDetect Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6)
Brand: MM

lyophilized, clone 5C6, Calbiochem^®

別名:

Anti-Vasodilator-stimulated Phosphoprotein, Activated

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この商品について

NACRES:

NA.41

UNSPSC Code:

12352203

Clone:

5C6, monoclonal

Species reactivity:

human, mouse

Application:

—

Citations:

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特性

biological source

mouse

Quality Segment

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

5C6, monoclonal

form

lyophilized

contains

≤0.1% sodium azide as preservative

species reactivity

human, mouse

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

dilution

(ELISA (0.05 µg/mL)
Immunoblotting (1 µg/mL, colorimetric; 0.1 µg/mL, chemiluminescence)
Immunocytochemistry (1-10 µg/mL)
Immunoprecipitation (1-10 µg/10 vanadate-treated A431 cells))

isotype

IgG1

shipped in

ambient

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer157)

Gene Information

human ... VASP(7408)

詳細

General description

Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the the ~50 kDa VASP protein phosphorylated at Ser¹⁵⁷.

Recognizes the the ~50 kDa VASP protein phosphorylated at Ser¹⁵⁷.

This PhosphoDetect Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) is validated for use in ELISA, Immunoblotting, ICC, IP for the detection of VASP (pSer¹⁵⁷).

Immunogen

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵⁷ phosphorylation site of human VASP

Application

ELISA (0.05 µg/ml)

Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)

Immunocytochemistry (1-10 µg/ml)

Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)

Physical form

Lyophilized from PBS, PEG, sucrose.

Preparation Note

Reconstitute in 1 ml sterile, dH₂O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.

Analysis Note

Positive Control
Vanadate-treated A431 cells

Other Notes

Bear, J.E., et al. 2001. Curr. Opin. Cell. Biol.13, 158.
Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol.166, 5550.
Burkhardt, M., et al. 2000. J. Biol. Chem.275, 33536.
Harbeck, B., et al. 2000. J. Biol. Chem.275, 40.
Smolenski, A., et al. 2000. J. Biol. Chem.275, 25723.

Does not cross-react with non-phosphorylated VASP or with unrelated phosphorylation sites. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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保管分類

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c