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688225

Caspase-1 Substrate VI, Fluorogenic

Name: Caspase-1 Substrate VI, Fluorogenic
Brand: MM

Fluorogenic caspase-1 substrate.

別名:

Caspase-1 Substrate VI, Fluorogenic, Z-YVAD-AFC

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この商品について

実験式（ヒル表記法）:

C₃₃H₃₆F₃N₅O₁₀

CAS番号:

219137-85-6

分子量:

719.66

MDL number:

MFCD01318881

UNSPSC Code:

12352200

NACRES:

NA.77

Assay:

98% (HPLC)

Form:

solid

Storage condition:

OK to freeze, desiccated, protect from light

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特性

Quality Segment

100

assay

98% (HPLC)

form

solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, desiccated, protect from light

color

white to off-white

solubility

DMSO: 5 mg/mL

fluorescence

λ_ex ~400 nm, λ_em ~505 nm

shipped in

ambient

storage temp.

−20°C

SMILES string

FC(F)(F)c1c2c([o][c](c1)=O)cc(cc2)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)C)Cc3ccc(cc3)O)C(C)C)C)CC(=O)O

InChI

1S/C33H36F3N5O10/c1-15(2)28(41-31(49)23(38-17(4)42)11-18-5-8-20(43)9-6-18)32(50)37-16(3)29(47)40-24(14-26(44)45)30(48)39-19-7-10-21-22(33(34,35)36)13-27(46)51-25(21)12-19/h5-10,12-13,15-16,23-24,28,43H,11,14H2,1-4H3,(H,37,50)(H,38,42)(H,39,48)(H,40,47)(H,41,49)(H,44,45)/t16-,23-,24-,28-/m0/s1

InChI key

GWKJISJKNKIJNP-DEQDFGQZSA-N

詳細

General description

Fluorogenic ICE substrate. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. AFC (7-amino-4-trifluoromethylcoumarin) based fluorometric substrates are superior to AMC (7-amino-4-methylcoumarin) substrates because released AFC has a greater Stoke′s shift than AMC. Excitation/emission maxima (nm) for AFC and AMC are 400/505 and 380/460, respectively.

Fluorogenic caspase-1 substrate. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Fluorogenic caspase-1 substrate

Product does not compete with ATP.

Reversible: no

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Ac-Tyr-Val-Ala-Asp-AFC

Jones, R.A., et al. 1998. Hepatology 27, 1632.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Walker, N.P., et al. 1994. Cell 78, 343.
Thornberry, N.A., et al. 1992. Nature 356, 768.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

保管分類

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c