69182
DNase I, RNase free
For applications in which maintenance of RNA integrity is critical
別名:
RNase-free DNase I
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この商品について
NACRES:
NA.85
UNSPSC Code:
12352202
form
liquid
manufacturer/tradename
Novagen®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
technique(s)
DNA extraction: suitable
input
general use sample(s)
suitability
suitable for nucleic acid purification
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
−20°C
General description
RNase-free DNase I digests either single- or double-stranded DNA, producing a mixture of mono- and oligonucleotides. Purified to be free of RNase, this preparation is qualified for applications in which maintenance of the integrity of RNA is critical. The enzyme selectively degrades DNA in the presence of RNA and can be used to remove DNA template following in vitro transcription reactions. This enzyme is also useful in other applications such as DNase footprinting and nick translation.
Other Notes
One unit will degrade 1 μg DNA in 10 minutes at 37°C. The reaction mixture (50 µl) contains 80 mM HEPES pH 7.5, 10 mM NaCl, 5 mM MgCl₂, 10 mM DTT, 1 µg plasmid DNA, and enzyme.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
保管分類
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
69182-ML: + 69182-3: + 69182-UN: + 69182-1000UN:
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