69522
T7•Tag® Monoclonal Antibody
別名:
T7 Tag Antibody
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この商品について
UNSPSC Code:
12352203
NACRES:
NA.41
Clone:
monoclonal
Species reactivity:
mouse
Application:
—
Citations:
-
clone
monoclonal
Quality Segment
species reactivity
mouse
manufacturer/tradename
Novagen®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG2b
shipped in
dry ice, wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
The T7-Tag® Antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as PEPscreen® and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select® Vectors). For increased versatility and sensitivity, this antibody is also available labeled with biotin and as a direct conjugate with alkaline phosphatase or horseradish peroxidase.
Biochem/physiol Actions
T7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal
Cross-reactivity: Negligible with bacterial, insect or mammalian cell lysates
Sensitivity: 1–10 ng (Western blot developed with chromogenic substrates) < 1 ng (AP or HRP conjugate developed with chemiluminescent substrates, or Biotin conjugate developed with Streptavidin AP and chromogenic substrates). Also suitable for immunoprecipitation and immunohistochemistry (1–3)
Working dilution- 1:5,000–1:10,000
Cross-reactivity: Negligible with bacterial, insect or mammalian cell lysates
Sensitivity: 1–10 ng (Western blot developed with chromogenic substrates) < 1 ng (AP or HRP conjugate developed with chemiluminescent substrates, or Biotin conjugate developed with Streptavidin AP and chromogenic substrates). Also suitable for immunoprecipitation and immunohistochemistry (1–3)
Working dilution- 1:5,000–1:10,000
Packaging
Plastic ampoule
Physical form
Stabilized solution of antibody in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein
Other Notes
- Krek, W., et al. 1994. Cell 78,, 161.
- Roberts, S.G.E. and Green, M. (1994) Nature 371, 717–720.
- Joneson, T., McDonough, M., Bar-Sag, D., and Van Aelst, L. (1996) Science 274, 1374–1376.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
PEPscreen is a registered trademark of Sigma-Aldrich Co. LLC
T7-Tag is a registered trademark of Merck KGaA, Darmstadt, Germany
T7SELECT is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Multiple Toxicity Values, refer to MSDS
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。