71545
BacMagic DNA Kit - Novagen
別名:
BacMagic-1 DNA Kit
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この商品について
NACRES:
NA.51
UNSPSC Code:
12352202
manufacturer/tradename
Novagen®
Quality Segment
storage condition
OK to freeze
shipped in
wet ice
storage temp.
2-8°C
General description
The BacMagic-1 DNA Kit provides a positive selection for creating baculovirus recombinants in preparation for generating recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification. BacMagic DNA is an AcNPV genome with a portion of the essential open reading frame (ORF) 1629 deleted, preventing nonrecombinant virus from replicating in insect cells. The target coding sequence in a compatible transfer plasmid, such as pIEx/Bac, pBAC, or pTriEx vectors, is cotransfected with BacMagic DNA into insect cells. Homologous recombination within the cells restores the function of the viral ORF1629. Only recombinant baculovirus can replicate, producing a homogeneous population of recombinants. The BacMagic™-1 DNA Kit contains the BacMagic-1 DNA, Insect GeneJuice® Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency.
Other Notes
- 0.5 µg- BacMagic DNA
- 50 µl- Insect GeneJuice Transfection Reagent
- 2 µg- Transfection Control Plasmid
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Multiple Toxicity Values, refer to MSDS
保管分類
12 - Non Combustible Liquids
引火点 (F)
Not applicable
引火点（°C）
Not applicable
一体何が起きているの？
WGK 2