AB1031
Anti-Aggrecan Antibody
CHEMICON®, rabbit polyclonal
別名:
Cartilage-specific proteoglycan core protein, Cartilage-specific proteoglycan core protein, aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122), aggrecan proteoglycan, aggrecan
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この商品について
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
mouse
Application:
immunofluorescence
immunohistochemistry
immunoprecipitation (IP)
western blot
immunohistochemistry
immunoprecipitation (IP)
western blot
Technique(s):
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
Citations:
189
Uniprot accession no.:
製品名
Anti-Aggrecan Antibody, Chemicon®, from rabbit
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse
manufacturer/tradename
Chemicon®
technique(s)
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ACAN(176)
Analysis Note
Control
Cartilage, neural tube, and brain tissue.
Cartilage, neural tube, and brain tissue.
Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis:
1:500 dilution of this lot detected AGGRECAN on 10 μg of Mouse Brain lysates.
Western Blot Analysis:
1:500 dilution of this lot detected AGGRECAN on 10 μg of Mouse Brain lysates.
Application
Anti-Aggrecan Antibody is an antibody against Aggrecan for use in IF, IH(P), IP & WB.
Immunohistochemistry(Paraffin):
A previous lot of this antibody was used at 10 μg/mL. See note under Protocol regarding triton X-100 treatments.
Immunoprecipitation:
A previous lot of this antibody was used at 5 μg/ 500 μL of cell membrane extract in PBS with inhibitors and >0.5% triton X-100.
A previous lot of this antibody was used at 10 μg/mL. See note under Protocol regarding triton X-100 treatments.
Immunoprecipitation:
A previous lot of this antibody was used at 5 μg/ 500 μL of cell membrane extract in PBS with inhibitors and >0.5% triton X-100.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Biochem/physiol Actions
AB1031 is specific for mouse aggrecan; other species have not been tested.
Expected to cross react with Human and rat.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Aggregating chondroitin sulfate proteoglycan or aggrecan, is one of the most studied proteoglycans. Many individual monomers of aggrecan bind to hyaluronic acid to form an aggregate, the monomer is known as aggrecan. The aggregates are comprised of up to 100 monomers attached to a single chain of hyaluronic acid. The high content of negatively charged polysaccharide chains contributes to the osmotic swelling of cartilage and in combination with intact collagen fibers results in articular cartilage resistance to compressive forces and high tensile strength. Aggrecan is abundant in articular cartilage and compromises 10% of its dry weight.
Immunogen
GST fusion protein containing amino acids 1177-1326 of mouse aggrecan.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
ImmunoAffinity Purified
Rabbit polyclonal antigen affinity purified antibody in PBS. Anti-GST has not been adsorbed. Liquid.
Note: Aggrecan is present in articular cartilage, brain, heart, and other tissues.
Note: Aggrecan is present in articular cartilage, brain, heart, and other tissues.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
AB1031:
jan
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Biglycan and fibromodulin have essential roles in regulating chondrogenesis and extracellular matrix turnover in temporomandibular joint osteoarthritis.
Embree, MC; Kilts, TM; Ono, M; Inkson, CA; Syed-Picard, F; Karsdal, MA; Oldberg, A; Bi, Y; Young, MF
The American Journal of Pathology null
Timothy P Rutkowski et al.
Journal of cell science, 129(11), 2145-2155 (2016-05-11)
RBPjκ-dependent Notch signaling regulates multiple processes during cartilage development, including chondrogenesis, chondrocyte hypertrophy and cartilage matrix catabolism. Select members of the HES- and HEY-families of transcription factors are recognized Notch signaling targets that mediate specific aspects of Notch function during
Huimin Yan et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(41), E6199-E6208 (2016-09-30)
Osteoarthritis (OA) is a major cause of disability and morbidity in the aging population. Joint injury leads to cartilage damage, a known determinant for subsequent development of posttraumatic OA, which accounts for 12% of all OA. Understanding the early molecular
Luigi A Nasto et al.
Mechanisms of ageing and development, 134(1-2), 35-42 (2012-12-25)
Intervertebral disc degeneration (IDD) is the leading cause of debilitating spinal disorders such as chronic lower back pain. Aging is the greatest risk factor for IDD. Previously, we demonstrated IDD in a murine model of a progeroid syndrome caused by
Aggrecan, link protein and tenascin-R are essential components of the perineuronal net to protect neurons against iron-induced oxidative stress.
Suttkus, A; Rohn, S; Weigel, S; Glockner, P; Arendt, T; Morawski, M
Cell Death & Disease null
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| AB1031 | 04053252623196 |
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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