AB1591P
Anti-Dopamine Transporter Antibody
Chemicon®, from rabbit
別名:
DAT
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この商品について
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA, IHC, IP, WB
Citations:
34
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... SLC6A3(6531)
General description
80 kDa
Plasmalemmal neurotransmitter transporters sequester synaptic and peri synaptic transmitter into presynaptic elements. The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. Levels of DAT protein expression are altered by chronic drug administration. The activity of the DAT reuptake carrier is sodium dependent, and it is suspected to play a role in such neurologic and psychiatric disorders as Parkinson′s disease, Tourette′s disease, schizophrenia, and addiction. It is a 12 transmembrane domain transporter with the N and C terminal regions located within the cytoplasm.
Immunogen
An 18 amino acid peptide sequence near the NH2-terminus of rat brain dopamine transporter coupled to KLH (Kilty et al. 1991; Giros et al. 1991; Shimada et al. 1991; Kitayma 1992). This region is presumably the cytoplasmic domain.
Application
Detect Dopamine Transporter using this Anti-Dopamine Transporter Antibody validated for use in ELISA, IH, IP & WB.
Western blot: 1-10 μg/mL using ECL. An antibody raised against a similar peptide has been shown to react with a broad 75-80 kDa band in rat striatum (Hastrup et al., 2001) and not cerebellum. Rat DAT contains various carbohydrate moieties and neuraminidase and N-Glycanase treatment reduces its molecular weight to about 55 kDa.
Immunoprecipitation: 2-10 μg of antibody (Chang et al., 2001)
Immunohistochemistry: 1:250-1:500 on frozen sections of tissues fixed with 4% paraformaldehyde (Chang et al., 2001; Ourednik, et al., 2002). 0.05% trypsin for 10-15min has been found effective in exposing the epitope in some preparations.
ELISA: 0.5-1.0 μg/mL using 1 μg/mL of DAT control peptide (Catalog Number AG296) per well.
Optimal working dilutions must be determined by end user.
Immunoprecipitation: 2-10 μg of antibody (Chang et al., 2001)
Immunohistochemistry: 1:250-1:500 on frozen sections of tissues fixed with 4% paraformaldehyde (Chang et al., 2001; Ourednik, et al., 2002). 0.05% trypsin for 10-15min has been found effective in exposing the epitope in some preparations.
ELISA: 0.5-1.0 μg/mL using 1 μg/mL of DAT control peptide (Catalog Number AG296) per well.
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Recognizes Dopamine transporter (DAT). By Western blot the antibody recognizes a broad 80 kDa band in rat striatum (Lew et al. 1992; Freed et al. 1995; Vaughan et al. 1996) and not cerebellum. Rat DAT contains various carbohydrate moieties and neuraminidase and N-Glycanase treatment reduces its molecular weight to about 55 kDa.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
保管分類
12 - Non Combustible Liquids
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
AB1591P:
jan
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Jian Huang et al.
Brain and behavior, 10(2), e01533-e01533 (2020-01-17)
This study aims to establish a methamphetamine (METH)-induced behavioral sensitization model using tree shrews, as well as to measure the protein expression of the dopamine D3 receptor (D3R) and dopamine transporter (DAT). Forty tree shrews were equally and randomly divided
Michael Y Aksenov et al.
Neurotoxicology, 29(6), 971-977 (2008-07-09)
Drug abuse is a risk factor for neurological complications in HIV infection. Cocaine has been shown to exacerbate HIV-associated brain pathology and enhance neurotoxicity of HIV-1 Tat and gp120 proteins. In this study, we found that the selective inhibitor of
Silke Frahm et al.
Behavioural brain research, 339, 153-168 (2017-11-29)
Alpha-Synuclein (α-Syn) accumulation is considered a major risk factor for the development of synucleinopathies such as Parkinson's disease (PD) and dementia with Lewy bodies. We have generated mice overexpressing full-length human α-Syn fused to a membrane-targeting signal sequence under the
Da Hee Jung et al.
eLife, 9 (2020-09-22)
Most therapeutic candidates for treating attention-deficit hyperactivity disorder (ADHD) have focused on modulating the dopaminergic neurotransmission system with neurotrophic factors. Regulation of this system by transcranial direct current stimulation (tDCS) could contribute to the recovery of cognitive symptoms observed in
Chisayo Kozuka et al.
Diabetologia, 60(8), 1502-1511 (2017-05-22)
Overeating of dietary fats causes obesity in humans and rodents. Recent studies in humans and rodents have demonstrated that addiction to fats shares a common mechanism with addiction to alcohol, nicotine and narcotics in terms of a dysfunction of brain
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| AB1591P | 04053252515811 |
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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