Anti-NG2, Alexa Fluor^™488 Conjugate Antibody

NG2 is a high molecular weight, integral membrane chondroitin sulfate proteoglycan. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells (i.e., O-2A progenitor cells). NG2 is also found on the surfaces of chondroblasts, proliferating capillary endothelial cells, some human melanoma cell lines, and on leukemic blasts in childhood acute lymphoblastic leukemia. The NG2 proteoglycan is likely to play a role in regulation of cell motility, axon outgrowth and the cellular responses to platelet-derived growth factor.

The unconjugated parent antibody (Cat. No. AB5320) has an observed molecular weight at ~270-300 kDa

Immunoaffinity purified NG2 Chondroitin Sulfate Proteoglycan from rat

Anti-NG2, Alexa Fluor^™488 Conjugate Antibody detects level of NG2 & has been published & validated for use in ICC & IHC.

Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected NG2 in adult rat brain tissue.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

This antibody identifies the intact proteoglycan and core protein by WB and ELISA. When oligodendrocyte precursor cells (i.e. O-2A progenitor cells) are stained alive, the stain appears as clusters on the cell surface. This antibody does not stain differentiated oligodendrocytes well.

Protein A adsorption

Purified rabbit polyclonal conjugated to Alexa Fluor^™ 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Control
Rat or Human oligodendrocyte precursor cells

Evaluated by Immunocytochemistry in rat oligodendrocyte precursor cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NG2 in rat oligodendrocyte precursor cells. Performed without Triton.

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