Anti-F-actin-capping protein subunit alpha-1 Antibody

F-actin capping protein subunit alpha (CapZ alpha) belongs to the F-actin capping protein family, which are characteristic heterodimers, consisting of an α subunit (31-36 kDa) and a β subunit (28-32 kDa), that cap the barbed end of actin filaments within all eukaryotes. Their ability to bind the actin filaments is in a manner independent of Ca2++ and requires the C-terminal end of both subunits for optimal binding. CapZ is contained within the Z-discs of striated muscle, where it functions to inhibit the polymerization and depolymerization of actin. Within vertebrates, there are three isoforms for each of CapZ’s subunits. All three alpha subunit isoforms are components of the WASH complex. Little is known about isoforms 1 and 2; however, isoform 3 is thought to be involved in spermatid morphogenesis and is exclusively observed within sperm and testis, with highest expression within the neck region of sperm.

~35 kDa

GST-tagged recombinant protein corres-ponding to human F-actin-capping protein subunit alpha-1.

Detect F-actin-capping protein subunit alpha-1 using this Anti-F-actin-capping protein subunit alpha-1 Antibody validated for use in WB & IC.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Western Blot (Snap i.d.) Analysis: 1 µg/mL from a previous lot detected F-actin-capping protein subunit alpha-1 on 10 µg of HeLa cell lysate.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected F-actin-capping protein subunit alpha-1 in NIH/3T3, HeLa, and A431 cells.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HeLa cell lysate

Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected F-actin-capping protein subunit alpha-1 on 10 µg of HeLa cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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