Anti-Collagen Type I Antibody

purified bovine type I collagen extracted from purified bovine skin.

Anti-Collagen Type I Antibody detects level of Collagen Type I & has been published & validated for use in ELISA, RIA, IH, IH(P).

Immunohistochemistry: 1:40 dilution for immunofluorescent staining of frozen bovine liver and skin tissues.

Immunohistochemistry on 2% formalin or PFA fixed, paraffin embedded tissues requires HEIR antigen retrieval and enhanced enzymatic detection; methyl-carnoy, acetone, or acid-aldehyde fixation can enhance reactivity for paraffin sections.

Radioimmunoassay

ELISA: 1:200 direct

Not recommended for Western blots.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Reacts with bovine collagen type I collagen.

Antibody shows less than 0.1% cross reactivity in RIA at 1:500 dilution with bovine collagen types II, IX & XI, or fibronectin; other collagens and ECM proteins untested.

Reactivity with human collagen type I <2.0% with RIA, 1:500; reactivity with Chicken collagen type I, less than 0.1% by RIA at 1:500 dilution.

Format: Purified

Protein G purified IgG fraction in liquid at 1 mg/mL in 0.01M phosphate, 0.09M NaCl, pH 7.2.No preservatives.

Maintain frozen at -20°C for up to 12 months. Avoid repeated freeze/thaw cycles

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: AB749

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.