Anti-Amphiphysin II Antibody

Amphiphysin is a nerve terminal-enriched protein thought to be involved in synaptic vesicle endocytosis. One of three amphiphysin homologs, amphiphysin II may interact with amphiphysin I in order to regulate synaptic vesicle endocytosis. During clathrin-mediated endocytosis, amphiphysin has been reported to bind to a component of the clathrin coat, clathrin assembly protein 2. Mutations in the expression of amphiphysin II may be causal to defects in the endocytosis pathway. Expression of amphiphysin II has been observed in neurons along with some endocrine cell types and spermatocytes.

It is well established (Ramjaun, 1997) that both Amphiphysin I and II run substantially slower on SDS-PAGE with a larger apparent molecular weight than the predicted molecular weights.

Epitope: Unknown

KLH-conjugated recombinant protein corresponding to human Amphiphysin II.

Detect Amphiphysin II using this Anti-Amphiphysin II Antibody validated for use in WB.

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

Western Blot Analysis: 1:1,000 dilution from a previus lot detected Amphiphysin II in 10 µg of rat brain and mouse brain membrane tissue lysates.

The antibody is raised against human amphiphysin II, but recognizes both amphiphysin I and Amphiphysin II (see Molecular Weight Notes).

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Mouse brain tissue lysate

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected Amphiphysin II in 10 µg of mouse brain tissue lysate.

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