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Merck

CP01

Anti-Actin (Ab-1) Mouse mAb (JLA20)

liquid, clone JLA20, Calbiochem®

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この商品について

NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
JLA20, monoclonal
Species reactivity:
-
Application:
—
Citations:
218

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biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

JLA20, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative (actin antibody only, CP01-100UL)

species reactivity (predicted by homology)

all

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

dilution

(Immunoblotting (1:5000-1:20000)
Immunofluorescence (1:10000,

Recomended dilution for secondary antibody (0.05 µg/mL, 1:2000 dilution))

isotype

IgM

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... ACTB(60)

General description

Anti-Actin (Ab-1), mouse monoclonal, clone JLA20, recognizes the ~ 42 kDa α-, β-, and γ isoforms of actin. It is validated for Western blotting and immunofluorescence.
Mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells supplied as ascites. Recognizes the ~42 kDa α-, β- and γ-isoforms of actin. Supplied with goat anti-mouse IgM conjugated to horseradish peroxidase.
Recognizes the ~42 kDa α-, β-, and γ isoforms of actin. Useful as a positive control antibody.
  • Antibody Target Gene Symbol: ACTA1, ACTB1, ACTG1
  • Target Synonym: AA959943, ACTA, Acta-2, Acts, Actsk-1, ALPHA SKELETAL ACTIN, ALPHA1 ACTIN, ASMA, CFTD, CFTD1, CFTDM, Fetal isoform of skeletal alpha-actin, G-actin, MPFD, NEM1, NEM2, NEM3, SKA, SKELETAL ALPHA-ACTIN, SKELETAL MUSCLE ACTIN
  • Entrez Gene Name: actin, alpha 1, skeletal muscle
  • Hu Entrez ID: 58, 71 (Related Antibodies: CP64)
  • Mu Entrez ID: 11459 ;-; 11465
  • Rat Entrez ID: 29437 ;-; 287876

    • Immunogen

    Chicken
    chicken cytoskeletal proteins

    Application

    Immunoblotting (1:5000-1:20000)

    Immunofluorescence (1:10000, see application references)



    Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)

    Physical form

    100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.

    Preparation Note

    Following initial thaw, aliquot ascites and freeze (-20°C).

    Analysis Note

    Positive Control
    All animal cell lines

    Other Notes

    Actin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.
    Lin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp.
    Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
    Lin, J.J. 1981. Proc. Natl. Acad. Sci.78, 2335.
    Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
    Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.

    Legal Information

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

    Disclaimer

    Toxicity: Multiple Toxicity Values, refer to MSDS (O)

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    保管分類

    11 - Combustible Solids

    wgk

    WGK 1

    flash_point_f

    Not applicable

    flash_point_c

    Not applicable

    適用法令

    試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

    CP01-1EA: + CP01-ML: + CP01-100UL: + US2CP01-1EA:

    jan

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    Peter Mullen et al.
    Clinical cancer research : an official journal of the American Association for Cancer Research, 10(6), 2100-2108 (2004-03-26)
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    American journal of physiology. Endocrinology and metabolism, 315(4), E676-E693 (2018-03-07)
    Obesity is associated with adipose tissue inflammation that contributes to insulin resistance. Zinc finger protein 36 (Zfp36) is an mRNA-binding protein that reduces inflammation by binding to cytokine transcripts and promoting their degradation. We hypothesized that myeloid-specific deficiency of Zfp36
    M C Hollander et al.
    Cell cycle (Georgetown, Tex.), 4(5), 704-709 (2005-04-23)
    Gadd45a is a p53-regulated gene whose protein product, like p53, is involved in maintenance of genome stability. Specifically, deletion of Gadd45a leads to extensive aneuploidy as a consequence of centrosome amplification and subsequent abnormal segregation of chromosomes during mitosis. S-phase
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    グローバルトレードアイテム番号

    カタログ番号GTIN
    CP01-1EACN07790788053697

    ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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