DP02
Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416)
liquid, clone PAb416, Calbiochem®
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この商品について
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
PAb416, monoclonal
Species reactivity:
SV40-infected cells
Application:
—
Citations:
38
biological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
PAb416, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
SV40-infected cells
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Affinity Purification
Immunoblotting (1-5 µg/mL)
Immunofluorescence (1-5 µg/mL)
Immunoprecipitation (1-5 µg antibody)
Paraffin Sections )
isotype
IgG2a
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~94 kDa SV40 large T antigen.
Recognizes ~94 kDa SV40 large T antigen in SV80 and SV-T2 cells.
SV40 T Ag (Ab-2) is a mouse monoclonal antibody with specificity for antigenic determinants unique to the SV40 large T antigen and non-reactive with SV40 small T antigen. Both antigens are encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular transforming protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the p105 product of the retinoblastoma susceptibility gene.
This Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) is validated for use in Affinity Purification, Immunoblotting, IF, IP, Paraffin Sections for the detection of SV40 T Antigen (Ab-2).
Immunogen
Epitope: Within amino acids 83-128
purified SV40 large T-antigen
Application
Affinity Purification (use Cat. No. DP02A)
Immunoblotting (1-5 µg/ml, see application references)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-5 µg antibody, use Cat. No. DP02A)
Paraffin Sections (see application references)
Immunoblotting (1-5 µg/ml, see application references)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-5 µg antibody, use Cat. No. DP02A)
Paraffin Sections (see application references)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Analysis Note
Negative Control
3T3 cells
3T3 cells
Positive Control
SV80 or SV-T2 cells
SV80 or SV-T2 cells
Other Notes
DeCaprio, J.A., et al. 1988. Cell54, 275.
Whyte, P., et al. 1988. Nature334, 124.
Mitchell, P.J., et al. 1987. Cell50, 847.
Dixon, R.A.F. and Nathans, D., 1985. J. Virol.53, 1001.
Simanis, V. and Lane, D.P. 1985. Virol.144, 88.
Mann, R.S. and Carroll, R.B. 1984. Virology138, 379.
Sarnow, P., et al. 1982. Cell28, 387.
Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA78, 41.
Lane, D.P. and Crawford, L.V. 1979. Nature278, 261.
Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA71, 3754.
Tooze, J. 1973. Cold Spring Harbor, New York.
Tegtmeyer, P. 1972. J. Virol.10, 591.
Whyte, P., et al. 1988. Nature334, 124.
Mitchell, P.J., et al. 1987. Cell50, 847.
Dixon, R.A.F. and Nathans, D., 1985. J. Virol.53, 1001.
Simanis, V. and Lane, D.P. 1985. Virol.144, 88.
Mann, R.S. and Carroll, R.B. 1984. Virology138, 379.
Sarnow, P., et al. 1982. Cell28, 387.
Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA78, 41.
Lane, D.P. and Crawford, L.V. 1979. Nature278, 261.
Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA71, 3754.
Tooze, J. 1973. Cold Spring Harbor, New York.
Tegtmeyer, P. 1972. J. Virol.10, 591.
The agarose conjugate (Cat. No. DP02A) is also available and suitable for affinity purification and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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保管分類
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
DP02-1MG: + DP02-200UG:
jan
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。