Anti-ABCA1 Antibody, clone AB.H10

Mutations in the ABCA1 gene are associated with Tangier disease (TD). TD is an autosomal recessive disorder results from an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and disorders in cellular lipid trafficking. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.

Recombinant fragment, corresponding to amino acids 1800-2260 of Human ABCA1.

ELISA
Immunohistochemistry (Paraffin): 5 μg/mL. Perform heat mediated antigen retrieval in sodium citrate buffer (pH 6), before commencing with IHC staining protocol. Amplification with biotin-streptavidin may be required.
Immunoprecipitation
Western Blot: 1 μg/mL. Predicted molecular weight: 254 kDa.

Optimal working dilutions must be determined by end user.

Research Category
Metabolism

Research Sub Category
Lipid Metabolism & Weight Regulation

This Anti-ABCA1 Antibody, clone AB.H10 is validated for use in ELISA, IP, WB, IH(P) for the detection of ABCA1.

Recognize specifically ABCA1 from human, mouse, and chicken.

Format: Purified

Presented as a liquid in PBS, pH 7.4 with 0.05% sodium azide.

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4°C for 3 months.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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