MAB1596
Anti-DLG4 Antibody
CHEMICON®, mouse monoclonal, 6G6-1C9
別名:
PSD-95
製品名
Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9, clone 6G6-1C9, Chemicon®, from mouse
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6G6-1C9, monoclonal
species reactivity
mouse, rat, bovine
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
bovine ... Dlg4(100137840)
mouse ... Dlg4(13385)
rat ... Dlg4(29495)
Other Notes
Replaces: 04-1066
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Purified immunoglobulin. Liquid in PBS, pH 7.2 with 50% glycerol and 0.09% sodium azide.
See datasheet for purification method.
Analysis Note
Control
Mouse brain, rat brain tissue.
Mouse brain, rat brain tissue.
Routinely evaluated in Immunocytochemistry.
Application
Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9 detects level of Post Synaptic Density Protein 95 & has been published & validated for use in IC, IH & WB.
Immunocytochemistry:
Representative image from a previous lot.
Confocal fluorescent analysis of PC12 using MAb1596 Mouse mAB (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue). Membrane staining positive.
Immunocytochemistry:
12.5 µg/mL
Western Blot:
1-5 μg/mL (ECL) on rat brain lysate (20 μg).
Optimal working dilutions must be determined by the end user.
Representative image from a previous lot.
Confocal fluorescent analysis of PC12 using MAb1596 Mouse mAB (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue). Membrane staining positive.
Immunocytochemistry:
12.5 µg/mL
Western Blot:
1-5 μg/mL (ECL) on rat brain lysate (20 μg).
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
Synapse & Synaptic Biology
Biochem/physiol Actions
Other species have not been tested.
Reacts with a protein of ~100 kDa, corresponding to the apparent molecular weight of PSD-95, on SDS-PAGE immunoblots of samples from rat, mouse and bovine. A slightly higher molecular weight band is also detected. In rat brain and mouse brain tissue extracts a band of ~75 kDa is also present.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
PSD-95 is part of a multiprotein complex restricted to the post-synaptic membrane. It is believed to initiate NMDA receptor clustering on the post synaptic membrane. Its restricted expression makes it an excellent marker for post-synaptic membrane regions on neurons.
~100 kDa
Immunogen
Recombinant rat PSD-95.
Preparation Note
Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Do not store below -20°C.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類
10 - Combustible liquids
wgk
WGK 2
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Ribbon synapse plasticity in the cochleae of Guinea pigs after noise-induced silent damage.
Shi, L; Liu, L; He, T; Guo, X; Yu, Z; Yin, S; Wang, J
Testing null
Ping He et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 26(1), 334-345 (2011-10-11)
The distribution of postsynaptic glutamate receptors has been shown to be regulated by proimmunocytokine tumor necrosis factor α (TNF-α) signaling. The role of TNF-α receptor subtypes in mediating glutamate receptor expression, trafficking, and function still remains unclear. Here, we report
Telencephalic neurons monosynaptically link brainstem and forebrain premotor networks necessary for song.
Roberts, TF; Klein, ME; Kubke, MF; Wild, JM; Mooney, R
The Journal of Neuroscience null
April P Neal et al.
Neurotoxicology, 32(2), 281-289 (2011-01-05)
N-methyl-D-aspartate receptor (NMDAR) ontogeny and subunit expression are altered during developmental lead (Pb²+) exposure. However, it is unknown whether these changes occur at the synaptic or cellular level. Synaptic and extra-synaptic NMDARs have distinct cellular roles, thus, the effects of
Transcriptome analysis of synaptoneurosomes identifies neuroplasticity genes overexpressed in incipient Alzheimer's disease.
Williams, C; Mehrian Shai, R; Wu, Y; Hsu, YH; Sitzer, T; Spann, B; McCleary, C; Mo, Y; Miller, CA
Testing null
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| MAB1596 | 04053252467301 |
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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