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Merck

MAB2510

Anti-Green Fluorescent Protein Antibody, original GFP only, clone 264-449-2

clone 264-449-2, Chemicon®, from mouse

別名:

GFP

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この商品について

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
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製品名

Anti-Green Fluorescent Protein Antibody, original GFP only, clone 264-449-2, clone 264-449-2, Chemicon®, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

264-449-2, monoclonal

species reactivity (predicted by homology)

all

packaging

antibody small pack of 25 μg

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

Analysis Note

Tested

Application

Anti-Green Fluorescent Protein Antibody, original GFP only, clone 264-449-2 is an antibody against Green Fluorescent Protein for use in IP, WB & IC.
Immunocytochemistry

Immunoblotting: 1:1000

Immunoprecipitation

Optimal working dilutions and protocols must be determined by the end user.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

Biochem/physiol Actions

Directed against a bacterially-expressed fusion protein. Also reacts with red-shifted GFP-variant. Does not react with enhanced GFP.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Immunogen

Epitope: original GFP only
highly-purified native GFP

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified
Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide.

Preparation Note

Store at 2-8°C for up to 12 months.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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保管分類

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


試験成績書(COA)

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以前この製品を購入いただいたことがある場合

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文書ライブラリにアクセスする

Fei Mao et al.
Acta neuropathologica, 142(4), 629-642 (2021-06-22)
The neurodegenerative diseases amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration with TAR DNA-binding protein-43 (TDP-43) inclusions (FTLD-TDP) share the neuropathological hallmark of aggregates of TDP-43. However, factors governing the severity and regional distribution of TDP-43 pathology, which may account
Guoqiang Cai et al.
The FEBS journal, 272(7), 1625-1638 (2005-03-30)
Neurotransmitter transporters play a major role in achieving low concentrations of their respective transmitter in the synaptic cleft. The GABA transporter GAT1 belongs to the family of Na(+)- and Cl(-)-coupled transport proteins which possess 12 putative transmembrane domains and three
Evangelia K Siska et al.
PloS one, 12(9), e0185498-e0185498 (2017-09-28)
Diabetes is a chronic disease characterized by high levels of blood glucose. Diabetic patients should normalize these levels in order to avoid short and long term clinical complications. Presently, blood glucose monitoring is dependent on frequent finger pricking and enzyme
Nguyet Nguyen et al.
Cell communication and signaling : CCS, 15(1), 48-48 (2017-11-16)
Human hyaluronic acid (HA) molecules are synthesized by three membrane spanning Hyaluronic Acid Synthases (HAS1, HAS2 and HAS3). Of the three, HAS1 is found to be localized more into the cytoplasmic space where it synthesizes intracellular HA. HA is a
Stamatia Laidou et al.
Redox biology, 32, 101458-101458 (2020-03-08)
Spinocerebellar ataxia type-1 (SCA1) is caused by an abnormally expanded polyglutamine (polyQ) tract in ataxin-1. These expansions are responsible for protein misfolding and self-assembly into intranuclear inclusion bodies (IIBs) that are somehow linked to neuronal death. However, owing to lack

グローバルトレードアイテム番号

カタログ番号GTIN
MAB251004053252396700
MAB2510-25UG04054839337482

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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