Anti-Integrin alpha 5 (ITGA5/CD49E) Antibody

Mouse embryonal carcinoma P19 cells.

Detect Integrin α5β1 using this Anti-Integrin α5β1 Antibody, clone BMC5 validated for use in FC, IP, IH.

Inhibition of cell adhesion to fibronectin: 10 μL/mL

Note that where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (alpha4beta1) integrin, is involved, inhibition of cell adhesion by BMC5 alone may not be complete.

Immunoprecipitation from lysate of 10E6 cell equivalent: 2 μL

Flow cytometry: 10μL/mL

Immunohistochemistry on acetone-fixed frozen sections only; formalin fixation is not recommended.

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

Antibody MAB2575 was raised against mouse integrin VLA-5 (alpha5beta1), a fibronectin receptor. The antibody was prepared by fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells. Specificity of the antibody was verified by its ability to immunoprecipitate alpha5beta1 heterodimers from (125)-I-surface labeled cells, reciprocal depletion of VLA-5 antigen from cell lysate with antiserum against the cytoplasmic domain of a5 integrin subunit, and immunoprecipitation of VLA-5 integrin from cells known to express this integrin. Cell lines tested to express alpha5beta1, include: P19 cells, mouse melanoma B16F1 cells, mouse squamous carcoma SCC cells and thymocytes. Characterization by immunoprecipitation was typically analyzed by SDS-PAGE using 6% gels under non-reducing conditions.

Liquid ascites.

Maintain frozen at -20°C in undiluted aliquots for up to 12 months.

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