Anti-Cardiac Troponin I Antibody, a.a. 87-91, clone 8E10

Epitope: a.a. 87-91

Free human cardiac troponin 1.

Detect Cardiac Troponin I using this Anti-Cardiac Troponin I Antibody, a.a. 87-91, clone 8E10 validated for use in ELISA & WB.

Research Category
Metabolism

Research Sub Category
Muscle Physiology

Western blotting: identifies two bands 14kDa and 18kDa which appear to be breakdown products of full length cTn1 (Morjana N. 1998, Degradation of human cardiac troponin I after myocardial infarction. Biotechnol Appl. Biochem. 28:105-11).

EIA: MAB3152 has been used successfully in ELISA and immunoaffinity purification. Its affinity constant is 8x10(10). It can be used with MAB3150 in sandwhich ELISA as the coating antibody.

Optimal working dilutions must be determined by end user.

MAB3152 reacts equally with free cardiac troponin 1 (cTn1) and cTn1 forming complexes with other troponin components. It recognizes epitopes that are not perturbed or sterically shielded by other troponin components. Not affected by heparin, phosphorylation, oxidation and complex formation. Recognizes an epitope located between amino acid residues 87 and 91 of cTnI. There is no cross-reaction with skeletal muscle troponin 1.

Format: Purified from hybridoma cell culture in bioreactor

Liquid in PBS (pH 7.4) with 0.1% sodium azide.

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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