MAB3418X
Anti-MAP2 Antibody, Alexa Fluor™ 488 conjugated
clone AP20, Chemicon®, from mouse
別名:
Anti-MAP-2, Anti-MAP2A, Anti-MAP2B, Anti-MAP2C
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この商品について
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
ALEXA FLUOR™ 488
Clone:
AP20, monoclonal
Application:
immunohistochemistry
Species reactivity:
rat, human, chicken, mouse, bovine
Citations:
20
Technique(s):
immunohistochemistry: suitable
Uniprot accession no.:
製品名
Anti-MAP2 Antibody, Alexa Fluor™ 488 conjugated, clone AP20, Chemicon®, from mouse
biological source
mouse
conjugate
ALEXA FLUOR™ 488
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
AP20, monoclonal
species reactivity
rat, human, chicken, mouse, bovine
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
chicken ... Map2(424001)
human ... MAP2(4133)
mouse ... Map2(17756), Map2(281294)
rat ... Map2(25595)
Analysis Note
Control
Brain, Neuronal culture, Human glioblastoma T98G cells
Brain, Neuronal culture, Human glioblastoma T98G cells
Application
Immunohistochemistry
Optimal working dilutions must be determined by end user.
APPLICATION NOTES:
Immunohistochemistry: Anti-MAP-2 can be used to stain tissue (brain or spinal cord) fixed with paraformaldehyde.
Optimal working dilutions must be determined by end user.
APPLICATION NOTES:
Immunohistochemistry: Anti-MAP-2 can be used to stain tissue (brain or spinal cord) fixed with paraformaldehyde.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Neurofilament & Neuron Metabolism
This Anti-MAP2 Antibody, Alexa Fluor 488 conjugated is validated for use in IH for the detection of MAP2.
Biochem/physiol Actions
Recognizes MAP-2 (microtubule associated protein-2). MAP-2 is one of several high molecular weight proteins that play an important role in brain microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles (1).
MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2 (2,3). MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds (4,5).
SDS-PAGE Profiles: In SDS-PAGE MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 300 kD. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier).
MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2 (2,3). MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds (4,5).
SDS-PAGE Profiles: In SDS-PAGE MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 300 kD. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier).
Disclaimer
Alexa Fluor™ is a registered trademark of Molecular Probes, Inc.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Bovine brain microtubule protein.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Protein A purified
Purified immunoglobulin conjugated to Alexa Fluor™ 488. Liquid in Phosphate buffer with 15 mg/mL BSA as a stabilizer and 0.1% sodium azide.
Preparation Note
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
MAB3418X:
jan
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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Microvascular failure is one of the key pathogenic factors in the dynamic pathological evolution after traumatic brain injury (TBI). Our laboratory and others previously reported that Annexin A2 functions in blood-brain barrier (BBB) development and cerebral angiogenesis, and recombinant human
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資料
Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| MAB3418X | 04053252580352 |
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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