MABE1056
Anti-ZBTB7A Antibody, clone 13E9
clone 13E9, from hamster(Armenian)
別名:
Zinc finger and BTB domain-containing protein 7A, Factor binding IST protein 1, FBI-1, Factor that binds to inducer of short transcripts protein 1, HIV-1 1st-binding protein 1, Leukemia/lymphoma-related factor, POZ and Krueppel erythroid myeloid ontogeni
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この商品について
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
13E9, monoclonal
Application:
IHC, WB
Citations:
-
biological source
hamster (Armenian)
Quality Segment
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
13E9, monoclonal
species reactivity
human, mouse
technique(s)
immunohistochemistry: suitable (paraffin), western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ZBTB7A(51341)
General description
Zinc finger and BTB domain-containing protein 7A (UniProt O95365; also known as Factor binding IST protein 1, Factor that binds to inducer of short transcripts protein 1, FBI-1, HIV-1 1st-binding protein 1, Leukemia/lymphoma-related factor, POK erythroid myeloid ontogenic factor, Pokemon, TIP21, POZ and Krueppel erythroid myeloid ontogenic factor, TTF-I-interacting peptide 21, Zinc finger and BTB domain-containing protein 7A, Zinc finger protein 857A) is encoded by the ZBTB7A (also known as FBI1, LRF, ZBTB7, ZNF857A) gene (Gene ID 51341) in human. Pokemon/ZBTB7A belongs to the family of POK (POZ/BTB and Krüppel) transcription factors that play important roles in cellular differentiation and oncogenesis. Pokemon/ZBTB7A overexpression is reported in cancers of various origins, including non–small-cell lung cancer (NSCLC), breast cancer, ovarian cancer, as well as in non-Hodgkin lymphoma, where it is found to directly repress the expression of the tumor suppressor ARF. On the other hand, loss of pokemon/ZBTB7A expression is also found to promote the progression of prostate tumorigenesis among a subset of human advanced prostate cancers by bypassing Pten loss–induced cellular senescence (PICS) as a result of Sox9-dependent oncogenic pathways activation.
~70 kDa observed. Target band appears larger than the calculated molecular weight of 61.4 kDa
Immunogen
Linear peptide corresponding to the N-terminal sequence of human ZBTB7A.
Application
Anti-ZBTB7A Antibody, clone 13E9 is an antibody against ZBTB7A for use in Western Blotting, Immunohistochemistry (Paraffin).
Western Blotting Analysis: 2.0 µg/mL from a representative lot detected ZBTB7A in 10 µg of HepG2 and K562 cell lysates.
Western Blotting Analysis: A representative lot detected much lower pokemon/ZBTB7A exprerssion levels in 6 human prostate cell lines when compared with the non-tumourigenic RWPE-1 prostate epithelial cell line (Wang, G., et al. (2013). Nat. Genet. 45(7):739-746).
Western Blotting Analysis: A representative lot detected a downregulated pokemon/ZBTB7A exprerssion in human DU145 prostate cancer cells transfected with miR-106b or miR-93, but not miR-25 (Wang, G., et al. (2013). Nat. Genet. 45(7):739-746).
Western Blotting Analysis: A representative lot detected pokemon/ZBTB7A expression in prostates from wild-type mice, but not transgenic mice with prostate-specific Zbtb7a gene-knockout (Wang, G., et al. (2013). Nat. Genet. 45(7):739-746).
Western Blotting Analysis: A representative lot detected pokemon/ZBTB7A expression in wild-type mouse thymocytes and mEFs, but not Zbtb7-/- mEFs. Clone13E9 also detected the overexpressed pokemon levels in thymic lymphomas developed among lckEµ-Pokemon transgenic mice (Maeda, T., et al. (2005). Nature. 433(7023):278-285).
Immunohistochemistry Analysis: A representative lot detected a positive correlation between the pokemon/ZBTB7A immunoreactivity and that of PTEN in formalin-fixed, paraffin-embedded human prostate cancer tissues (Wang, G., et al. (2013). Nat. Genet. 45(7):739-746).
Immunohistochemistry Analysis: A representative lot detected nuclear pokemon/ZBTB7A immunoreactivity in human (thymus and tonsil) and mouse (thymus and spleen) lymphoid organs using paraffin-embedded tissue sections (Maeda, T., et al. (2005). Nature. 433(7023):278-285).
Immunohistochemistry Analysis: A representative lot detected specific nuclear pokemon/ZBTB7A immunoreactivity only in Zbtb7+/+ fetal liver (FL) cells, but not in Zbtb7-/- FL cells (Maeda, T., et al. (2005). Nature. 433(7023):278-285).
Immunohistochemistry Analysis: A representative lot detected pokemon/ZBTB7A immunoreactivity among subsets of human T-cell lymphomas, diffuse large B-cell lymphoma (DLBCL), and follicular lymphoma (FL) samples, while little pokemon immunoreactivity was seen in normal human thymic CD3+ cells (Maeda, T., et al. (2005). Nature. 433(7023):278-285).
Western Blotting Analysis: A representative lot detected much lower pokemon/ZBTB7A exprerssion levels in 6 human prostate cell lines when compared with the non-tumourigenic RWPE-1 prostate epithelial cell line (Wang, G., et al. (2013). Nat. Genet. 45(7):739-746).
Western Blotting Analysis: A representative lot detected a downregulated pokemon/ZBTB7A exprerssion in human DU145 prostate cancer cells transfected with miR-106b or miR-93, but not miR-25 (Wang, G., et al. (2013). Nat. Genet. 45(7):739-746).
Western Blotting Analysis: A representative lot detected pokemon/ZBTB7A expression in prostates from wild-type mice, but not transgenic mice with prostate-specific Zbtb7a gene-knockout (Wang, G., et al. (2013). Nat. Genet. 45(7):739-746).
Western Blotting Analysis: A representative lot detected pokemon/ZBTB7A expression in wild-type mouse thymocytes and mEFs, but not Zbtb7-/- mEFs. Clone13E9 also detected the overexpressed pokemon levels in thymic lymphomas developed among lckEµ-Pokemon transgenic mice (Maeda, T., et al. (2005). Nature. 433(7023):278-285).
Immunohistochemistry Analysis: A representative lot detected a positive correlation between the pokemon/ZBTB7A immunoreactivity and that of PTEN in formalin-fixed, paraffin-embedded human prostate cancer tissues (Wang, G., et al. (2013). Nat. Genet. 45(7):739-746).
Immunohistochemistry Analysis: A representative lot detected nuclear pokemon/ZBTB7A immunoreactivity in human (thymus and tonsil) and mouse (thymus and spleen) lymphoid organs using paraffin-embedded tissue sections (Maeda, T., et al. (2005). Nature. 433(7023):278-285).
Immunohistochemistry Analysis: A representative lot detected specific nuclear pokemon/ZBTB7A immunoreactivity only in Zbtb7+/+ fetal liver (FL) cells, but not in Zbtb7-/- FL cells (Maeda, T., et al. (2005). Nature. 433(7023):278-285).
Immunohistochemistry Analysis: A representative lot detected pokemon/ZBTB7A immunoreactivity among subsets of human T-cell lymphomas, diffuse large B-cell lymphoma (DLBCL), and follicular lymphoma (FL) samples, while little pokemon immunoreactivity was seen in normal human thymic CD3+ cells (Maeda, T., et al. (2005). Nature. 433(7023):278-285).
Biochem/physiol Actions
Clone 13E9 detected pokemon/ZBTB7A expression in wild-type, but not Zbtb7-/-, mouse embryonic fibroblasts by Western blotting and showed strong and specific nuclear staining only in Zbtb7+/+ fetal liver (FL) cells, but not in Zbtb7-/- FL cells (Maeda, T., et al. (2005). Nature. 433(7023):278-285).
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected ZBTB7A in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected ZBTB7A in 10 µg of HeLa cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
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保管分類
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
MABE1056:
jan
試験成績書（COA）
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