Anti-Ago1 Antibody, clone 4B8

Protein argonaute-1 (UniProt Q9UL18; also known as Argonaute1, Argonaute RISC catalytic component 1, eIF-2C 1, eIF2C 1, Eukaryotic translation initiation factor 2C 1, hAgo1, Putative RNA-binding protein Q99) is encoded by the AGO1 (also known as EIF2C, EIF2C1) gene (Gene ID 26523) in human. Ago1 belongs to the family of argonaute proteins involved in gene expression by regulating gene transcription and RNA splicing in the nucleus, as well as by regulating posttranscriptional gene silencing mediated by siRNAs and microRNAs in the cytosol. Ago1 is shown to interact with chromatin modifiers, splicing factors, and RNA polymerase II. Ago1 and the related Ago2 are also shown to target Hepatitis C Virus (HCV) RNÁ 5′-untranslated region (5′-UTR) in an miR-122-dependent manner, resulting in viral RNA stability and translation stimulation.

~97 kDa observed

GST-tagged recombinant human Ago1.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Ago1 in 10 µg of K562 cell lysate.
Western Blotting Analysis: A representative lot detected Ago1-containing messenager ribonucleoprotein (mRNP) complexes primarily in the low density fractions of sucrose gradient-fractionated HEK293 lysate (Höck, J., et al. (2007). EMBO Rep. 8(11):1052-1060).
Immunoprecipitation Analysis: Representative lots immunoprecipitated FLAG-tagged Ago1, but not FLAG-tagged Ago2, Ago3, or Ago4 (Ender, C., et al. (2008). Mol. Cell. 32(4):519-528; Beitzinger, M., et al. (2007). RNA Biol. 4(2):76-84).
RNA Binding Protein Immunoprecipitation (RIP): Representative lots co-immunoprecipitated Ago1-associated RNAs, including mRNAs, miRNAs, and snoRNAs from human cell lysates (Li, Z., et al. (2009). J. Virol. 83(24):12751-12758; Ender, C., et al. (2008). Mol. Cell. 32(4):519-528; Beitzinger, M., et al. (2007). RNA Biol. 4(2):76-84).

Clone 4B8 sepcifically immunoprecipitated FLAG-tagged Ago1,but not FLAG-tagged Ago2, Ago3, or Ago4 (Ender, C., et al. (2008). Mol. Cell. 32(4):519-528).

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Ago1 in 10 µg of HeLa cell lysate.

Concentration: Please refer to lot specific datasheet.

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