Anti-Replication Protein A Antibody, clone RPA34-19

RPA (RP-A p32) is a heterotrimeric protein complex that binds specifically to single-stranded DNA (ssDNA). It is composed of three subunits: RPA1 (70 kDa), RPA2 (32 kDa), and RPA3 (14 kDa). RPA plays multiple roles in DNA replication. At the onset of DNA replication, RPA is loaded onto chromatin, and is needed for subsequent loading of DNA polymerase and other replication proteins to initiate DNA replication. After replication begins, RPA moves with replication forks, stabilizing ssDNA and assisting in DNA synthesis. In addition to its replication function, RPA is also known to play essential roles in damage repair and recombination. The 32 kDa subunit is phosphorylated by the cdc2 family of kinases when cells enter S-phase; and by ATM, ATR, and DNA-PK proteins in response to DNA damage.

~34 kDa observed. This protein has 3 isoforms: Isoform 1 (~29 kDa), Isoform 2 (~30 kDa), and Isoform 3 (~39 kDa).

Replication Protein A purified from U293 cells.

Anti-Replication Protein A Antibody, clone RPA34-19 is a Mouse Monoclonal Antibody for detection of Replication Protein A also known as Replication protein A 32 kDa subunit, RP-A p32 & has been validated in WB, ICC & IHC.

Immunocytochemistry Analysis: A 1:250 dilution from a reprsentative lot detected Replication Protein A in A431 cells.

Immunohistochemistry Analysis: A 1:5 dilution from a representative lot detected Replication Protein A in human placental chorionic villi and in human colorectal adenocarcinoma tissue.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HeLa cell lysate

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: A 1:2,000 dilution of this antibody detected Replication Protein A in 10 µg of HeLa cell lysate.

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