MABE350
Anti-phospho-RNAPII (Tyr1) Antibody, clone 3D12
clone 3D12, from rat
別名:
DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1
ログインで組織・契約価格をご覧ください。
サイズを選択してください
表示を変更する
この商品について
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
3D12, monoclonal
Species reactivity:
human
Application:
ChIP, IP, WB
Citations:
1
biological source
rat
Quality Segment
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3D12, monoclonal
species reactivity
human
technique(s)
ChIP: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pTyr1)
General description
RNA polymerase II (RNAPII or Pol II) is a multi-subunit enzyme responsible for the transcription of transcription of DNA into RNA from protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. RNAPII can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
~220 kDa observed
Immunogen
Ovalbumin-conjugated linear peptide corresponding to human RNAPII phosphorylated at Tyr1.
Application
Detect the phospho-RNAPII (Tyr1) protein using this Anti-phospho-RNAPII (Tyr1), clone 3D12 validated for use in WB, IP & ChIP.
Immunoprecipitation Analysis: A representative lot from an independent laboratory detected phospho-RNAPII (Tyr1) in proliferating yeast whole cell extract (Mayer, A., et al. (2012). Science. 336(6089):1723-1725.).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot from an independent laboratory immunoprecipitated phospho-RNAPII (Tyr1) in proliferating yeast whole cell extract (Mayer, A., et al. (2012). Science. 336(6089):1723-1725.).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot from an independent laboratory immunoprecipitated phospho-RNAPII (Tyr1) in proliferating yeast whole cell extract (Mayer, A., et al. (2012). Science. 336(6089):1723-1725.).
Physical form
Format: Purified
Analysis Note
Control
Untreated and lambda phosphatase treated HeLa cell lysates
Untreated and lambda phosphatase treated HeLa cell lysates
Evaluated by Western Blot Analysis in untreated and lambda phosphatase treated HeLa cell lysates.
Western Blot Analysis: 2 µg/mL of this antibody detected phospho-RNAPII (Tyr1) in 10 µg of untreated HeLa cell lysate. (Lambda phosphatase treatment was performed during the western blot procedure.)
Western Blot Analysis: 2 µg/mL of this antibody detected phospho-RNAPII (Tyr1) in 10 µg of untreated HeLa cell lysate. (Lambda phosphatase treatment was performed during the western blot procedure.)
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Still not finding the right product?
製品選択ツール}で選択肢を絞り込んでください。
保管分類
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable