Anti-VE-Cadherin Antibody (CD144), clone BV9

88 kDa calculated

Also known as CD144, human Vascular Endothelial (VE)-cadherin is a calcium-dependent adhesion molecule strictly located at cell to cell junctions. VE-cadherin is present in all types of endothelium (veins, arteries, capillary and large vessels). Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Cadherins are reported to play an important role in angiogeneis and in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha catenin forming a link to the cytoskeleton.

Epitope: Extracellular domain.

Recombinant protein corresponding to the extracellular domain of human VE-Cadherin.

Activity Assay Analysis: A representative lot from an independent laboratory increased endothelial permeability, inhibited the organization of vasclular-like structures, and prevented the protective effect of VEGF against apoptosis in endothelial cells.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

This VE-Cadherin antibody is validated for use in ICC & Enzyme Assay for the detection of the VE-Cadherin protein.

This antibody recognizes the extracellular domain of VE-Cadherin.

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing PBS without 0.05% sodium azide.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
HUVEC cells

Evalutated by Immunocytochemistry in HUVEC cells.

Immunocytochemistry Analysis: 1 µg/mL of this antibody detected VE-Cadherin in HUVEC cells.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.