NA03
Anti-PCNA (Ab-1) Mouse mAb (PC10)
liquid, clone PC10, Calbiochem®
別名:
Anti-Proliferating Cell Nuclear Antigen
製品名
Anti-PCNA (Ab-1) Mouse mAb (PC10), liquid, clone PC10, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
PC10, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rat, yeast, mouse, human, zebrafish
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Immunoblotting (2.5 µg/mL)
Immunofluorescence (1-5 µg/mL)
Immunoprecipitation (not recommended with unconjugated antibody)
Paraffin Sections (2.5 µg/mL, no pre-treatment required))
isotype
IgG2a
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PCNA(5111)
zebrafish ... Pcna(30678)
Analysis Note
Positive Control
HeLa cells, HL-60 cells, tonsil tissue, or normal colon tissue
HeLa cells, HL-60 cells, tonsil tissue, or normal colon tissue
Application
Immunoblotting (2.5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (not recommended with unconjugated antibody, see comments)
Paraffin Sections (2.5 µg/ml, no pre-treatment required, see comments)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (not recommended with unconjugated antibody, see comments)
Paraffin Sections (2.5 µg/ml, no pre-treatment required, see comments)
Disclaimer
Toxicity: Standard Handling (A)
General description
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0-Ag14 mouse myeloma cells. Recognizes the ~37 kDa PCNA protein.
Recognizes the ~37 kDa PCNA protein in HeLa cells as well as in tonsil or normal colon tissue.
This Anti-PCNA (Ab-1) Mouse mAb (PC10) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation Paraffin Sections for the detection of PCNA (Ab-1).
Immunogen
Epitope: Within amino acids 112-121
recombinant PCNA
Other Notes
Staining shows an intense nuclear pattern. Not recommended for immunoprecipitation because in some samples a ~37 kDa protein will be precipitated that is not PCNA. Generally, no pre-treatment is needed to stain paraffin sections, but staining may be enhanced in some samples by pretreating with 4 N HCl for 10 min at 37°C followed by 3 washes in water. Antibody should be titrated for optimal results in individual systems.
Waseem, N.H. and Lane, D.P. 1990. J. Cell. Sci.96, 121.
Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA86 3189.
Bravo, R., et al. 1987. Nature (London)326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol.105, 1549.
Miyachi, K., et al. 1987. J. Immunol.121, 2228.
Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA86 3189.
Bravo, R., et al. 1987. Nature (London)326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol.105, 1549.
Miyachi, K., et al. 1987. J. Immunol.121, 2228.
Packaging
Please refer to vial label for lot-specific concentration.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| NA03-200UGCN | 04055977227642 |
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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