PC223L
Anti-Neuropeptide Y Rabbit pAb
lyophilized, Calbiochem®
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この商品について
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
human, avian, amphibian, fish, feline, rat, newt
Application:
—
Citations:
-
biological source
rabbit
Quality Segment
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
contains
≤0.1% sodium azide as preservative
species reactivity
human, avian, amphibian, fish, feline, rat, newt
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Frozen Sections (1:1000-1:2000 for free-floating, PAP detection))
isotype
IgG
shipped in
ambient
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... NPY(4852)
General description
Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes neuropeptide Y.
Recognizes neuropeptide Y in cortex, caudate, putamen, and paraventricular nucleus.
This Anti-Neuropeptide Y Rabbit pAb is validated for use in Frozen Sections for the detection of Neuropeptide Y.
Immunogen
porcine neuropeptide Y
Application
Frozen Sections (1:1000-1:2000 for free-floating, PAP detection)
Preparation Note
Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Analysis Note
Positive Control
Paraventricular nucleus, cortex, or caudate putamen
Paraventricular nucleus, cortex, or caudate putamen
Other Notes
Antibody specificity was examined in the rat cortex, caudate putamen, and paraventricular nucleus. Staining is completely eliminated by pretreatment of antibody with neuropeptide Y (10-6M). Preabsorption with avian pancreatic polypeptide, β-endorphin, cholecystokinin, peptide YY, somatostatin, or vasoactive intestinal peptide resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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保管分類
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable