PMEF-NL-P1
EmbryoMax® Primary Mouse Embryonic Fibroblasts
PMEF, Neo Resistant, Strain FVB, Not Treated, Passage 1
別名:
Mouse Embryonic Fibroblasts
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この商品について
UNSPSC Code:
41106509
NACRES:
NA.75
eCl@ss:
32011203
biological source
mouse
Quality Segment
manufacturer/tradename
Specialty Media, EmbryoMax®
technique(s)
cell culture | stem cell: suitable
input
sample type primary embryotic fibroblasts (PMEFs)
sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)
shipped in
liquid nitrogen
storage temp.
-140 to -196°C
General description
PMEF-NL-P1 are low passage neomycin resistant Primary Mouse Embryo Fibroblasts (PMEFs) isolated from FVB mouse embryos at day 13. The cells have NOT been mitotically arrested. PMEFs are the traditional feeder cells used to support the growth of undifferentiated mouse and human pluripotent stem cell cultures. Additionally, low passage primary cultures of fibroblasts have a high proliferative capacity and can be used for reprogramming. It is known that cellular reprogramming efficiency declines with each passage, presumably due to lowered proliferative capacity of PMEFs at later passages. These untreated PMEF cells are high quality, low passage (P1) mouse fibroblasts that have been successfully reprogrammed into iPS cells with high efficiency. They can be used to study the mechanisms of reprogramming or be further expanded and treated with γ-irradiation or mitomycin-C to make growth arrested feeder cells. See Murine Embryonic Stem Cell Culture Manual for complete culture and media for PMEF cells.
The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.
Application
Research Category
Stem Cell Research
Stem Cell Research
Biochem/physiol Actions
Primary Mouse Embryo Fibroblasts
Packaging
1x106 cells
Physical form
Provided frozen in EMD Millipore Freezing Medium (S-002-D) containing DMSO.
Preparation Note
After receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.
Legal Information
EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Substances Subject to be Indicated Names
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Substances Subject to be Notified Names
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試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。