Dextran Sulfate Solution

Dextran Sulfate (MW >500,000) is used as an additive in probe hybridization solutions. Its presence accelerates the rate of hybridization by 10 fold. The addition of Dextran Sulfate favors the formation of probe networks, resulting in greater sensitivity during membrane-bound nucleic acid analysis. The product is supplied as a 50% (w/v) liquid.

Dextran Sulfate - 50% Solution

To prepare a solution containing 10% Dextran Sulfate, add 8 ml of hybridization ingredients to 2 ml of 50% Dextran Sulfate Solution.



See the "Hybrisol Nomograph for Dextran Sulfate" link located under the "Protocols" tab at catalog number S4031 product specific page on www.chemicon.com for determining the proper concentration of dextran sulfate needed for various hybridization condition guidelines.

Research Category
All

A 50% dextran sulfate solution in water filtered through a 20 micron membrane and autoclaved prior to bottling in clean, sterilized bottles containing no preservatives. Liquid solution is dark brown from autoclaving.

Store at room temperature tightly sealed when not in use. Solution can be stored refrigerated however it should be warmed to at least room temperature prior to use dilution for best results.

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