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11759779001

Roche

BM Chemiluminescence ELISA Substrate (AP)

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UNSPSC Code:
12352204
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usage

sufficient for 1,000 tests (ELISA), sufficient for 600 tests (SEAP)

packaging

pkg of 150 mL

manufacturer/tradename

Roche

shipped in

wet ice

storage temp.

2-8°C

Biochem/physiol Actions

CSPD is dephosphorylated by alkaline phosphatase (AP). The resulting instable dioxetane anion decomposes and emits light at a maximum wavelength of 477nm. Chemiluminescence-enhancing reagents improve the quantum yield of the excited state by more than 500 fold.
The AP assay system is designed for measuring alkaline phosphatase of various sources (e.g. intestine, placenta, milk, bacteria).

Features and Benefits

  • Sensitive: Approximately 10fg intestinal alkaline phosphatase can be detected
  • High dynamic measuring range: Linear range of more than five orders of magnitude
  • Constant light emission: The assay produces a long-lasting light emission instead of a short peak kinetics

Preparation Note

Working solution: Preparation of working solution

The substrate reagent is composed of AP-substrate (bottle 1), Enhancer (bottle 2) and Assay Buffer (bottle 3). Prepare substrate reagent freshly before use. The ratio of the components depends on the application. The substrate reagent is stable at least for one day at 2 to 8 °C.

Other Notes

1. Alkaline Phosphatase Substrate, CSPD
2. Enhancer, Emerald II
3. Assay Buffer
For life science research only. Not for use in diagnostic procedures.

保管分類

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

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