製品名
抗FLAG® M2抗体 アフィニティーゲル, purified immunoglobulin, buffered aqueous glycerol solution
conjugate
agarose conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
M2, monoclonal
form
buffered aqueous glycerol solution
analyte chemical class(es)
proteins
technique(s)
affinity chromatography: suitable
immunoprecipitation (IP): suitable
matrix
(4% agarose bead; 45-165μm bead size)
isotype
IgG1
capacity
>0.6 mg/mL, resin binding capacity (FLAG-BAP)
shipped in
wet ice
storage temp.
−20°C
Quality Level
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Application
製品の詳細につきましては、FLAG®アプリケーションポータルをご覧ください。
Disclaimer
General description
溶出 - FLAG®ペプチド、グリシン、pH 3.5、3x FLAG®ペプチド
Immunogen
Physical form
Legal Information
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保管分類
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
資料
The FLAG® Expression System is a proven method to express, purify and detect recombinant fusion proteins. Sigma®, the proven provider of FLAG®, now offers a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions. The ANTI-FLAG® M2 Magnetic Bead is composed of murine derived, anti-FLAG® M2 monoclonal antibody attached to superparamagnetic iron impregated 4% agarose beads, with an average diameter of 50 µm. The M2 antibody is capable of binding to fusion proteins containing a FLAG peptide sequence at the N-terminus, Met-N-terminus, or C-terminus locations in mammalian, bacterial, and plant extracts.
プロトコル
M2モノクローナル抗体4%アガロースアフィニティーゲルを使用したFLAG®融合タンパク質の免疫沈降(IP)のためのプロトコル
Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels
関連コンテンツ
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.
イオン交換、サイズ排除、タンパク質アフィニティークロマトグラフィーなどの方法を用いた組換えタンパク質精製のためのタンパク質精製技術、試薬、プロトコル。
EZviewTM Red Protein A and ANTI-FLAG® M2 Affinity Gels: Immunoprecipitation with Enhanced Visibility Affinity Beads - Technical Article - July 2001
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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