B5897
Anti-Bak antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
別名:
Anti-Bcl-2 Homologous Antagonist/Killer
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この商品について
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, IHC (p), WB
Citations:
32
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 28 kDa
species reactivity
human
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using sections of human colon carcinoma, microarray: suitable, western blot: 1:2,000 using human epidermal carcinoma A431 whole cell extract
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... BAK1(578)
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関連するカテゴリー
General description
Bak (Bcl-2 homologous antagonist/killer, Bak1) belongs to the B-cell lymphoma 2 (Bcl-2) family of proteins. The bak gene is mapped to chromosome 6 and encodes a 233 amino acid protein with a predicted MW of 23.4 kDa. Bak shares homology with Bcl-2 in the Bcl‐2 homology (BH) domains (BH1 and BH2). Bak is expressed in a wide variety of cell types and tissues, with the highest levels observed in heart and skeletal muscle.
Immunogen
synthetic peptide corresponding to the N-terminus of human Bak amino acids 23-38 with C-terminally added lysine, conjugated to KLH.
Application
Anti-Bak antibody produced in rabbit has been used in immunoblotting and immunohistochemistry.
Biochem/physiol Actions
Bak (Bcl-2 homologous antagonist/killer, Bak1) is involved in regulating apoptosis. Bak can accelerate the rate of apoptosis when overexpressed in some cell lines. Increased Bak expression in normal and neoplastic intestinal epithelial cells results in apoptosis. However, expression of Bak in a human lymphoblastoid cell line, provided protection from apoptosis induced by serum deprivation and the oxidant menadione, suggesting that the function of Bak may be context dependent.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
IXO11034: + B5897-BULK: + B5897-VAR: + B5897-.2ML:
jan
Up regulation of Bax and down regulation of Bcl2 during 3-NC mediated apoptosis in human cancer cells
Naseri MH, et al.
Cancer Cell International, 15(1), 55-55 (2015)
Association of Bax and Bak Homo-oligomers in Mitochondria Bax REQUIREMENT FOR Bak REORGANIZATION AND CYTOCHROMEc RELEASE
Mikhailov V, et al.
Test, 278, 5367-5376 (2003)
mRNA for N-Bak, a neuron-specific BH3-only splice isoform of Bak, escapes nonsense-mediated decay and is translationally repressed in the neurons
Jakobson M, et al.
Cell Death & Disease, 3(2), e269-e269 (2012)
Stephen Ma et al.
The Journal of biological chemistry, 288(36), 26027-26038 (2013-07-31)
Bak and Bax are the essential effectors of the intrinsic pathway of apoptosis. Following an apoptotic stimulus, both undergo significant changes in conformation that facilitates their self-association to form pores in the mitochondrial outer membrane. However, the molecular structures of
Rachel T Uren et al.
eLife, 6 (2017-02-10)
During apoptosis, Bak and Bax undergo major conformational change and form symmetric dimers that coalesce to perforate the mitochondrial outer membrane via an unknown mechanism. We have employed cysteine labelling and linkage analysis to the full length of Bak in
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