Human Collagen Type I

コラ-ゲンは構造や遺伝子が異なる多数のタイプに分類されています。シグマでは、BornsteinとTraubが提唱した命名法を使用しています。シグマのタイプ分けは一般的なコラ-ゲン分類とは異なりますのでご注意ください。

Collagen type 1 alpha 2 (COL1A2) encodes pro-alpha2 chain and is a component of heterodimer, type 1 collagen fiber. It is mapped to human chromosome 7q21.3. COL1A2 associates with COL1A1 in the ratio 1:2 and undergoes posttranslational modification to form mature type I collagen fibre.

I型コラーゲンは、広範な生体材料工学研究において、in vitroで幹細胞を増殖させる骨格として利用されてきました。

Human collagen has been used:

• as a component of extracellular matrix in the chemotaxis assay of the rat adipose-derived stem cells
• in adhesion assay of the adult retinal pigmented epithelium-19 (ARPE-19) cell line
• in the glycation aggregation and adsorption studies as a model system for arthritis

Collagen type 1 alpha 2 (COL1A2) is crucial for bone formation, cartilage and blood vessels. Imbalance in COL1A2 may be the cause for dental fluorosis. Missense mutations involving glycine substitutions in the COL1A2 gene alters the collagen triple helix structure decreasing its stability and is implicated in osteogenesis imperfecta. Mutations near the splice site of COL1A2 gene results in exon skipping and is associated with Ehlers-Danlos Syndrome. An insertion or deletion polymorphism in the COL1A2 gene impairs its interaction with microRNA and modulates the bone mineral density resulting in high susceptibility to osteoporosis.

Gallop, P.M.の方法の変法に従い、ヒトの皮膚から調製されています。