C7594
カゼインブロッキングバッファー
for Northern and Southern blotting, powder blend
別名:
blocking buffer
General description
Casein is a milk-derived substance routinely used in blocking buffers for Western, Southern and Northern Blot procedures. Blocking agents are used to reduce non-specific binding of detection reagents to the membranes.
Application
Suitable as a blocking Buffer for Western, Southern and Northern Blot membranes. Blocked membranes can then be used in either chemiluminescent, colorimetric, or DIG detection assays.
Features and Benefits
- Better blocking performance than dried milk or BSA
- Compatible with neutral and positively-charged membranes
- Compatible with chemiluminescent, colorimetric , and DIG detection assays
Preparation Note
Dissolve entire contents of bottle in 800 mL molecular biology grade water. Stir until well mixed, then add water to final volume of 1L. Store at 2-8°C for up to one week.
保管分類
11 - Combustible Solids
wgk
WGK 1
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
C7594-BULK: + C7594-VAR: + C7594-1L:
jan
Tadatsura Koshika et al.
Immunology, 134(4), 386-397 (2011-11-03)
α1,3-Galactosyltransferase gene-knockout pigs transgenic for porcine cytotoxic T-lymphocyte antigen 4 immunoglobulin (pCTLA4-Ig) have been produced to reduce T-cell-mediated rejection following xenotransplantation. The level of soluble pCTLA4-Ig in their blood was greatly in excess of the therapeutic level in patients, rendering
Ochratoxin A in some French wines: application of a direct competitive ELISA based on an OTA-HRP conjugate.
Radoi, A., et al.
Analytical Letters, 42, 1187-1187 (2009)
Serapion Pyrpassopoulos et al.
Methods in molecular biology (Clifton, N.J.), 1046, 389-403 (2013-07-23)
Many proteins that reside in the cytoplasm bind directly to cell membranes and play roles in signaling, adhesion, metabolism, cell structure, and cell motility. Several of these membrane-binding proteins, especially cytoskeletal proteins, have mechanical functions that result in the transmission
Elizabeth R Sunderhaus et al.
Frontiers in neuroscience, 13, 1207-1207 (2019-11-30)
Mutations in patatin-like phospholipase domain-containing protein 6 (PNPLA6) have been linked with a number of inherited diseases with clinical symptoms that include spastic paraplegia, ataxia, and chorioretinal dystrophy. PNPLA6 is an evolutionary conserved protein whose ortholog in Drosophila is Swiss-Cheese
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
プロトコル
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.
関連コンテンツ
Instructions
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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