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この商品について
UNSPSC Code:
41106514
Biological source:
mouse blood
Growth mode:
Suspension
Karyotype:
Not specified
Morphology:
Lymphoblast
biological source
mouse blood
packaging
tube of 5 μg 93042610-DNA-5UG, pkg of vial of cells 93042610-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
CTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines.
Biochem/physiol Actions
CTLL is a subclone of T cells derived from a C57bl/6 mouse. The cells require IL-2 for growth and are used to assay for its presence in conditioned media.
Mouse C57b1/6 T cell
Preparation Note
Maintain cultures between 3-9 x 105 cells/ml; 5% CO2; 37°C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 4-5 x 105 cells/ml.
RPMI 1640 + 2mM Glutamine + 10-20 IU/ml Human Recombinant IL-2 + 10% Foetal Bovine Serum (FBS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.