CHOZN^® Elite Cell Line

The CHOZN^® Elite cell line is a subclone of our original CHOZN^®ZFN-Modified CHO Cell Line (CHOGS-1VL) that grows in suspension culture with chemically defined and animal component-free media. It is a glutamine synthetase (GS) knock out rendering the cells auxotrophic for the essential amino acid L-glutamine, which enables fast and easy selection of clones.

CHOZN^®Elite cell line enables high producing clones, achieving a titer of 7-13 g/L with the standard vector, resulting in cost savings. For an easy adoption, the cell line is plug-and-play with your established CLD processes, for use in both fed-batch and perfusion processes. The cell line enables highly stable clones (85% of clones maintain ≥70% of the titer over 60 generations), a consistent and predictable growth profile (from 18 to 24 x 10⁶ cells/mL for fed-batch) and regulatory compliance (cGMP banked, adventitious agent tested with full traceability and documentation available).

Cells are provided in vials containing 1 mL at 10 x 10⁶ cells/mL. Cells are cGMP banked in EX-CELL^® CD CHO Fusion Medium (with 6 mM L-glutamine) and 7% DMSO.

A license agreement must be acquired prior to use of the cell line, or any products derived from the cell line in a clinical or commercial process. CHOZN is a registered trademark of Merck KGaA, Darmstadt, Germany.

CHOZN is a registered trademark of Merck KGaA, Darmstadt, Germany

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany